Extraction method of wheat genome DNA

Abstract

The invention discloses an extraction method of a wheat genome DNA. The method comprises the following steps: putting wheat material samples to be extracted into 96-well plates respectively; then putting steel balls into each hole; uncovering and putting into a vacuum freezing dryer to be subjected to freeze drying for 20-28 hours at the temperature of -100-(-120) DEG C; grinding the wheat material samples by using a grinding instrument; finally, extracting DNA from the grinded powder samples by using a discharge gun in an improved SDS method. According to the extraction method, wheat tissue vanes are pulverized by using the grinding instrument after vacuum freeze drying is carried out; compared with a traditional liquid nitrogen grinding method, the extraction method has the advantages that the labor force is greatly released, and the working efficiency is improved; meanwhile, by using 96-well plates and the discharge gun, the sample extraction efficiency is greatly improved. The extraction method of the wheat genome DNA is convenient, fast, high in flux and high in quality.

Description

technical field [0001] The invention relates to a plant genome DNA extraction method, in particular to a wheat genome DNA extraction method. Background technique [0002] With the continuous development of molecular biology, the demand for samples in many molecular biology experiments is very large. Simple, fast, efficient and stable DNA extraction technology is a necessary prerequisite for promoting the development of wheat-related research at the molecular level, and it is also the key to research efficiency. The DNA extraction of the wheat genome generally needs to go through several steps such as breaking the cell wall, lysing the cell membrane, removing secondary substances such as polysaccharides and polyphenols, denaturing and separating proteins, precipitating nucleic acids, removing RNA, and concentrating DNA. Generally speaking, the CTAB (cetyltriethylammonium bromide) method has unique advantages in processing plant tissues containing polysaccharide components, an...

AI Technical Summary

Problems solved by technology

However, the traditional CTAB method is cumbersome to operate, consumes a lot of reagents, and uses many utensils. The grinding utensils need to be reused, which is easy to cause sample contamination and low yield.

[0003] At present, most of the reports on plant DNA extraction methods are improved on the basis of SDS method and CTAB method, but none of them can get rid of the operation steps of grinding samples one by one with grinding rods or glass rods, and the extraction steps are cumbersome and time-consuming.

In recent years, other simple extraction methods of plant DNA have also appeared, such as high salt and low pH value method, alkali treatment method, high temperature water boiling method, etc. Although these methods are simple in steps, fast in speed and low in cost, avoid using liquid nitrogen, phenol, etc. Reagents, but there are often many problems such as poor DNA extraction quality, about 300 samples can be extracted a day, and cannot meet the higher requirements of PCR amplification.

Images