Extraction method of wheat genome DNA

An extraction method and genome technology are applied in the field of wheat genome DNA extraction, which can solve the problems of cumbersome and time-consuming extraction steps, poor DNA extraction quality, and complicated operations, simplifying the steps of DNA extraction, and improving the efficiency of sample addition and extraction. The effect of work efficiency
CN104694530AInactive Publication Date: 2015-06-10NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2015-06-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an extraction method of a wheat genome DNA. The method comprises the following steps: putting wheat material samples to be extracted into 96-well plates respectively; then putting steel balls into each hole; uncovering and putting into a vacuum freezing dryer to be subjected to freeze drying for 20-28 hours at the temperature of -100-(-120) DEG C; grinding the wheat material samples by using a grinding instrument; finally, extracting DNA from the grinded powder samples by using a discharge gun in an improved SDS method. According to the extraction method, wheat tissue vanes are pulverized by using the grinding instrument after vacuum freeze drying is carried out; compared with a traditional liquid nitrogen grinding method, the extraction method has the advantages that the labor force is greatly released, and the working efficiency is improved; meanwhile, by using 96-well plates and the discharge gun, the sample extraction efficiency is greatly improved. The extraction method of the wheat genome DNA is convenient, fast, high in flux and high in quality.
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Description

technical field

[0001] The invention relates to a plant genome DNA extraction method, in particular to a wheat genome DNA extraction method. Background technique

[0002] With the continuous development of molecular biology, the demand for samples in many molecular biology experiments is very large. Simple, fast, efficient and stable DNA extraction technology is a necessary prerequisite for promoting the development of wheat-related research at the molecular level, and it is also the key to research efficiency. The DNA extraction of the wheat genome generally needs to go through several steps such as breaking the cell wall, lysing the cell membrane, removing secondary substances such as polysaccharides and polyphenols, denaturing and separating proteins, precipitating nucleic acids, removing RNA, and concentrating DNA. Generally speaking, the CTAB (cetyltriethylammonium bromide) method has unique advantages in processing plant tissues containing polysaccharide components, an...

Claims

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