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Lucid ganoderma fermentation method

A Ganoderma lucidum fermentation and fermenter technology, which is applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of low content of Ganoderma lucidum triterpenoids, and achieve the effect of simple method and increased yield

Active Publication Date: 2015-06-10
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the liquid fermentation technology of Ganoderma lucidum has made great progress, but at present, in the liquid fermentation process of Ganoderma lucidum, most

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The preserved strains were first transferred to a slant medium, placed in an incubator at 26°C for 10 days, and then fermented in shake flasks for 7 days at a temperature of 26°C and a rotation speed of 100rpm. Finally, submerged fermentation was carried out in a 6L fermenter for 140 hours. The submerged fermentation conditions were: : The ventilation rate is 6L / min, the temperature is 26°C, and the constant stirring speed is 100rpm during the fermentation process.

[0017] After 140 hours of fermentation, the content of Ganoderma lucidum mycelium was determined to be 7.66g / L, the yield of extracted Ganoderma lucidum triterpenes was 0.052mg / 100mg, and the production intensity of Ganoderma lucidum triterpenes was 2.84×10 -5 g / (L·h).

[0018] Wherein the determination method of Ganoderma lucidum triterpenes is:

[0019] After the ganoderma lucidum mycelium obtained by fermentation is ground into powder, 1 g of the mycelium is added to 50 ml of 95% ethanol for leaching fo...

Embodiment 2

[0022] Carry out the ganoderma lucidum fermentation according to the method of embodiment 1, just change the constant stirring speed in the submerged fermentation process to 150rpm, measure the ganoderma lucidum mycelia content 8.77g / L after the end of fermentation 140h, the ganoderma lucidum triterpene output is 1.020mg / 100mg, ganoderma lucidum three Terpene Production Strength 6.39×10 -4 g / (L h) (measurement method is the same as embodiment 1).

Embodiment 3

[0024] Carry out the ganoderma lucidum fermentation according to the method of embodiment 1, just control the stirring speed at 150rpm during 0-40h in the submerged fermentation process, and control the stirring speed at 100rpm after 40h. The terpene yield was 5.520mg / 100mg, and the production intensity of Ganoderma lucidum triterpenoids was 4.72×10 -3 g / (L h) (measurement method is the same as embodiment 1).

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Abstract

The invention discloses a lucid ganoderma fermentation method. The method comprises the following steps: firstly, shifting a strain to a slant medium and cultivating in an incubator at a temperature ranging from 25 to 32 DEG C for 7-14 days; secondly, fermenting in a shake flask for 7-10 days at a temperature ranging from 25 to 32 DEG C and a rotating speed ranging from 80 to 200rpm; finally, performing deep fermentation in a fermentation tank for 100-160 hours, wherein the deep fermentation is performed under the conditions that a ventilation rate ranges from 4 to 8L/min, a temperature ranges from 25 to 32 DEG C, and a stirring rotating speed is controlled within the range of 120-180rpm in 0-40 hours of fermentation and a stirring rotating speed is controlled within the range of 80-120rpm 40 hours later in the fermentation process. Due to the two stages of stirring rotating speed control processes, the yield of the ganoderma triterpenes can be remarkably improved.

Description

technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a ganoderma fermentation method. Background technique [0002] Ganoderma lucidum (Ganoderma lucidum) is a fungus belonging to Basidiomycetes, Polyporaceae, and Ganoderma genus. It is the most famous higher medicinal fungus in my country. The key medicinal components of Ganoderma lucidum are polysaccharides and triterpenes of Ganoderma lucidum. Among them, Ganoderma lucidum triterpenes have anti-inflammatory, analgesic, sedative, detoxifying, liver-protecting, anti-HIV, anti-tumor, liver-protecting effects, inhibit histamine release, angiotensin-converting enzyme and other functions, and are an important material basis for Ganoderma lucidum to exert its medicinal effects. [0003] At present, the yield of Ganoderma lucidum and its key medicinal components has become a bottleneck hindering the application of Ganoderma lucidum products. Since the production of Ganoderma lu...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12R1/645
Inventor 冯杰张劲松冯娜贾薇杨焱刘方刘艳芳唐传红吴迪颜梦秋唐庆九周帅张忠庄海宁
Owner SHANGHAI ACAD OF AGRI SCI