Probe for detecting K-ras gene mutation, primer, kit and method

Abstract

The invention discloses a probe for detecting K-ras gene mutation. The probe is Taqman MGB fluorescence probe in specific binding with a mutant type sequence of the 13th code of K-ras gene. The invention further discloses a primer and a kit for detecting K-ras gene mutation, the kit comprises the Taqman MGB fluorescent probe in specific binding with the mutant type sequence of the 13th code of the K-ras gene, a primer pair for amplifying the nucleotide sequence of the 13th code containing the K-ras gene; and PNA designed aiming at the 13th code GCC of the wild type K-ras gene. The invention further discloses a dual-fluorescent mark probe real-time quantitative detection method in K-ras gene mutation. The kit and the detection disclosed by the invention are capable of qualitatively and quantitatively detecting the mutation of the 13th code of K-ras gene at the same time, the sensitivity is high, the operation is simple and convenient, and wide application prospect is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology and is applied to biological science research and clinical molecular diagnosis, in particular to a probe, primer, kit and method for detecting the mutation of the 13th codon of K-ras gene. Background technique [0002] K-ras gene (GenBank: NC_000012) mutations are expressed in a variety of cancers, such as pancreatic cancer, colon cancer, blood cancer, thyroid cancer, bile duct cancer, lung cancer, endometrial cancer, ovarian cancer, skin cancer, spermatogonia Among them, K-ras gene mutation plays an important role in the occurrence and development of pancreatic cancer and colon cancer. It has been reported in the literature that point mutations at codon 12, 13 or 61 of the K-ras gene can be detected in pancreatic cancer tissue, pancreatic juice or feces, blood, and colon cancer tissue. Studies have shown that the detection of K-ras gene mutation in somatic DNA can be used as a method for early diagn...

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the technical problems that the current methods for detecting K-ras gene mutations cannot be qualitative and quantitative at the same time, the operation is cumbersome, and the sensitivity is low, and a probe, primer, and reagent for detecting the mutation of the 13th codon of the K-ras gene are provided. Box and method, the method can determine the mutation type and mutation amount of the 13th codon of K-ras gene by one PCR reaction, and the operation is simple and sensitive

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