BRCA gene susceptibility SNP locus detection composition

A composition and gene technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of complex operation, false positive, cumbersome operation, etc., and achieve high sensitivity, high specificity, detection High throughput effect

Active Publication Date: 2015-06-10
JIANGSU MICRODIAG BIOMEDICINE TECH CO LTD
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] At present, the commonly used BRCA gene mutation detection methods mainly include the following: Fluorescent quantitative PCR technology, which has high sensitivity, specificity, and high degree of automation, but there will be sample contamination and high false positives, and each time only Can detect a type
Restriction small fragment length polymorphism analysis (RFLP) is used to detect genes with altered restriction sites, and can directly determine the genotype, but it cannot be used for the detection of genes witho

Method used

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  • BRCA gene susceptibility SNP locus detection composition
  • BRCA gene susceptibility SNP locus detection composition
  • BRCA gene susceptibility SNP locus detection composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1: BRCA gene mutation screening of breast cancer patients

[0082] 1. Experimental materials

[0083] 1. The 20 clinical blood samples involved in this experiment came from the First Affiliated Hospital of Zhejiang University School of Medicine. After the samples were collected, they were immediately stored in a refrigerator at -80 degrees.

[0084] 2. The purity of all primers should be up to electrophoresis level (PAGE) or HPLC level, free of miscellaneous bands. Provide the quality inspection certificate of the synthesized product issued by the synthesis organization, such as PAGE electrophoresis result or HPLC analysis chart, which proves that there should be an obvious single-peak PAGE or HPLC purification chart after PAGE or HPLC purification, and the concentration is 10ng / μl for use.

[0085] 3. All reagents were purchased from regular manufacturers: multiplex PCR enzyme (KAPA company), Ampure magnetic beads (Beckman Coulter company), PGM Template OT2kit, PGM Se...

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Abstract

The invention discloses a BRCA gene susceptibility SNP locus detection composition. The BRCA gene susceptibility SNP locus detection composition comprises a primer of SEQ NO:1-SEQ NO:448. The BRCA gene susceptibility SNP locus detection composition has the advantages that more than two thousand susceptibility SNP loci of a BRCA gene can be detected at one time, for the susceptibility SNP loci relative to breast cancer and ovarian cancer, the detection throughput is high, the specificity is strong, the pollution is not prone to occur, and the safety is high, the detection result has good accuracy and repeatability, and certain auxiliary diagnosis and implication effects are achieved for susceptible population of the breast cancer and the ovarian cancer, especially for crowd with family heredity of the two cancers.

Description

Technical field [0001] The invention relates to the field of gene detection, in particular to a composition for detecting the SNPA site of the BRCA gene. Background technique [0002] Breast cancer is one of the most common malignant tumors that seriously affect women's health and even endanger their lives. According to statistics, its incidence accounts for 7-10% of various malignant tumors throughout the body, which is second only to uterine cancer in women. The incidence of breast cancer is often related to genetics. In addition, the incidence of breast cancer is higher in women between 40-60 years of age and before and after menopause. [0003] Breast cancer can be divided into two categories: sporadic and hereditary. Among them, hereditary breast cancer accounts for about 5%-10% of the incidence of breast cancer. Breast cancer susceptibility genes BRCA1 (the first familial breast and ovarian cancer susceptibility gene) and BRCA2 (the second familial breast cancer susceptibi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 张利清官民晓刘蕊芳焦淑静王弢何素莉
Owner JIANGSU MICRODIAG BIOMEDICINE TECH CO LTD
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