Large-scale preparation method of 1/4 MS (containing carbon sources and fixed media) basic solid culture matrix
A fixed medium and production method technology, applied in the field of plant biology, can solve problems such as precipitation, mother liquor infection of bacteria, mold, and easy calculation errors
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[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this embodiment, a large-scale production method of a 1 / 4MS (containing carbon source and fixed medium) basic solid culture substrate includes five steps: accurate calculation, accurate weighing, mixing first and then more, sealing, and unsealing. step.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 20 elements contained in the basic solid base of 1 / 4MS (including carbon source and fixed medium) by 1000 times, that is, 0.025g each of cobalt chloride hexahydrate and copper sulfate pentahydrate, and 0.1g of thiamine hydrochloride , sodium molybdate dihydrate 0.25g, nicotinic acid 0.5g, pyridoxine hydrochloride 0.5g, potassium iodide 0.83g, glycine 2g, boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, ethylenediamine tetrahydrate Sodium iron acetate 36.5g, potas...
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