Scale production method of high-accuracy MS culture substrate
A technology for cultivating substrates and manufacturing methods, applied in the field of plant biology, can solve the problems of different colors, instrument precision errors, affecting test results, etc., and achieve the effects of high precision and small weighing errors
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[0013] The present invention will be further described below in conjunction with specific embodiments.
[0014] In this example, a large-scale production method of high-precision MS (containing carbon source and fixed medium) solid culture substrate, including accurate calculation, accurate weighing, first mixing less and then more, sealing, separate mother liquor preparation, unpacking and using Wait for six steps.
[0015] The specific operation of the above six steps is as follows:
[0016] ① Accurate calculation: expand the 16 elements contained in the MS basic medium by 1000 times, namely 0.1g thiamine hydrochloride, 0.25g sodium molybdate dihydrate, 0.5g nicotinic acid, 0.5g pyridoxine hydrochloride, and 0.83g potassium iodide , glycine 2g, boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, sodium iron edetate 36.5g, inositol 100g, potassium dihydrogen phosphate 170g, magnesium sulfate heptahydrate 370g, dihydrate Calcium chloride 44...
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