The invention discloses a large-scale production method for a B5 (containing a
carbon source)
solid culture medium, belonging to the field of
plant biotechnologies. The large-scale production method comprises the following steps: firstly, calculating the demanded quantities of 18 elements contained in the B5 (containing the
carbon source)
solid culture medium after 1000 times of amplification; accurately weighing the amount of the amplified
medicine, wherein CuSO4.5H2O and CoCl2.6H2O need to be accurately weighed by a one over ten-thousand balance; sequentially mixing the 18 elements according to the demanded quantities, namely, mixing the two fewest elements at first, and then adding the second fewest elements and uniformly mixing, and the like; sealing per 250g of the
solid culture medium in a solid plastic container; and unsealing for use as needed respectively. The B5 (containing the
carbon source) solid culture medium after 1000 times of amplification can be prepared through the method, has the characteristics of being convenient to operate, rapid, simple to apply, and having small errors of different batches and in the batches, avoids the phenomena of large errors,
precipitation, mildewing and the like caused by the influences of manual operations of calculation, weighing and the like, and external factors such as weather, temperature and
water content during preparation for a B5
liquid culture medium, as well as can be applied to biotechnological application of
plant tissue culture and the like.