62results about How to "No calculation error" patented technology

The invention discloses a large-scale preparation method of a high-precision 1/2MS (containing carbon sources and fixed media) culture medium, belonging to the plant biotechnology field. The large-scale preparation method comprises the following steps: firstly computing the quantity demands after 18 elements contained in the medium are increased by 1000 times and the quantity demands after CuSO4.5H2O and CoCl2.6H2O with minimal quantity demands are increased by 10000 times; accurately weighing chemicals after increase; successively mixing the 18 elements uniformly according to the quantity demands and sealing a solid medium; dissolving CuSO4.5H2O and CoCl2.6H2O in the quantity demands with 1L of distilled water for later use; and respectively unsealing the solid medium as needed to be used. The large-scale preparation method has the beneficial effects that the medium prepared by the method has the characteristics of high precision, convenience and quickness in operation, simplicity in application and small errors between different batches and in the batches; the phenomena such as large errors, precipitation and mouldiness, caused by manual operations and impacts of external factors, are avoided; the method can be applied to the biotechnology such as plant tissue culture.

The invention discloses a large-scale manufacturing method of high-precision MT (containing carbon source) culture matrices and belongs to the technical field of plant biology. The large-scale manufacturing method comprises the following steps: firstly calculating the demand quantity of 15 elements contained in the matrices after amplifying by 1000 times and the demand quantity of CuSO4.5H2O and CoCl2.6H2O in the very small demand quantity after amplifying by 10000 times; accurately weighing medicines; sequentially mixing and sealing the 15 elements according to the demand quantity; dissolving CuSO4.5H2O and CoCl2.6H2O in 1L of distilled water for later use; and respectively unsealing and using according to needs. The matrices which can be prepared by the method have characteristics of high precision, convenience in operation, high speed, simple application and small errors between different batches and in the same batch; and furthermore, the phenomena of great errors, precipitation, mildewing and the like caused by influence of manual operation and external factors are avoided, and the method can be applied to plant tissue culture and other biotechnical applications.

The invention discloses a large-scale production method for a high-accuracy B5 culture medium, belonging to the field of plant biotechnology. The large-scale production method comprises the following steps: firstly, calculating the demanded quantities of 15 elements contained in the medium after 1000 times of amplification and the demanded quantities of CuSO4.5H2O and CoCl2.6H2O after 10000 times of amplification; accurately weighing the amplified medicine; sequentially mixing and sealing the 15 elements according to the demanded quantities respectively; dissolving CuSO4.5H2O and CoCl2.6H2O by 1L of distilled water for the later use; and unsealing as needed respectively. The high-accuracy B5 culture medium prepared by the method is convenient to operate, rapid, simple to apply, and has small errors of different batches and in the batches, avoids the phenomena of large errors, precipitation, mildewing and the like caused by the influences of manual operations and external factors, as well as can be applied to biotechnological application of plant tissue culture and the like.

The invention discloses a large-scale preparation method for an improved Nitsch basic solid culture matrix, and belongs to the field of plant biotechnology. The method comprises the following steps: firstly, calculating the demand quantity of 15 elements, amplified by 1,000 times, in the matrix; secondly, accurately weighing amplified drug quantity, wherein CuSO4.5H2O and Na2MoO4.2H2O need to be accurately weighed by a one ten-thousandth balance; thirdly, mixing the 15 elements according to the demand quantity successively and sealing; finally, unsealing for use according to the needs respectively. The matrix prepared with the method has the characteristics of convenience for operation, quickness, simplicity for application, different batches and small errors in the batches, avoids the phenomena of big errors, deposition, mildewing and the like caused by influence of manual operations of calculating, weighing and the like as well as external factors of weather, temperature, moisture and the like during preparation of a Nitsch liquid culture matrix, and can be applied to biotechnologies of plant tissue culture and the like.

The invention discloses a method for preparing an MT (mounting medium-and-carbon source containing) solid culture medium in a large scale and belongs to the technical field of plant biology. The method for preparing the MT (mounting medium-and-carbon source containing) solid culture medium in the large scale comprises the following steps: firstly calculating demanded quantities of eighteen components contained in the MT (mounting medium-and-carbon source containing) solid culture medium after being expanded by 1000 times; accurately weighing expanded quantities of medicaments, wherein CuSO4.5H2O and CoCl2.6H2O are accurately weighed by utilizing a ten-thousandth balance; successively mixing the eighteen components according to gradually increasing demanded quantities, namely the two components with the lowest demanded quantities are mixed firstly, then the component with the next-to-the-lowest demanded quantity is added, uniform mixing is carried out, and the process is repeated; sealing every 250g of solid culture medium in one fixed plastic container; and opening the plastic containers respectively as required during application. The method for preparing the MT (mounting medium-and-carbon source containing) solid culture medium in the large scale has the advantages that the MT (mounting medium-and-carbon source containing) solid culture medium expanded by 1000 times can be prepared, the characteristics of easiness and speediness in operation, simplicity in application, small error in different batches and small intra-batch error can be realized, and the phenomena, such as big error, precipitation and moulding, which are caused by manual operations such as calculating and weighing and external factors such as weather, temperature and moisture during preparation of MT liquid culture medium can be avoided, so that the method for preparing the MT (mounting medium-and-carbon source containing) solid culture medium in the large scale can be applied to biotechnical uses such as plant tissue culture.

The invention discloses a large-scale preparation method of an MT (carbon source-contained) solid culture medium, and belongs to the technical field of plant biologics. The large-scale preparation method comprises the following steps: firstly, calculating the demand amounts of 17 elements of the MT (carbon source-contained) solid culture medium after being amplified for 1000 times; accurately weighing the amplified amounts of medicines; sequentially uniformly mixing the 17 elements according to the demand amounts, and sealing the MT (carbon source-contained) solid culture medium; unsealing to use the solid culture medium according to demands. The method can prepare the MT (carbon source-contained) solid culture medium, has the characteristics of being convenient and rapid to operate, simple to use and small in errors of different batches and same batches, prevents the phenomena of large errors, deposition, mildew and the like caused by artificial operation and external factors, and can be applied to biological technical purposes such as plant tissue culture.

The invention discloses a massive preparation method of a high-precision MS (Murashige & Skoog) (containing carbon source) culture substrate, and belongs to the technical field of plant biology. The method comprises the following steps: calculating 1000-times expanded quantity demand of 17 elements contained in the substrate and 10000-times expanded quantity demand of CuSO4.5H2O and CoCl2.6H2O under extremely small quality demand; accurately weighting drug quantity after expanding; sequentially mixing the 1000-times expanded 17 elements according to the quantity demand from small to large; sealing; dissolving CuSO4.5H2O and CoCl2.6H2O with 1L of distilled water for later use; respectively opening to use as required. According to the method, the prepared substrate is high in precision; the method has the characteristics of being convenient and fast to operate, simple to apply, and small in error of different batches and the same batch, and is applicable to plant tissue culture and other biological applications.

The invention discloses a large-scale manufacturing method of high-precision C17 (containing fixed medium) culture matrices and belongs to the technical field of plant biology. The large-scale manufacturing method comprises the following steps: firstly calculating the demand quantity of 17 elements contained in the matrices after amplifying by 1000 times and the demand quantity of CuSO4.5H2O in the very small demand quantity after amplifying by 10000 times; accurately weighing medicines; sequentially mixing and sealing the 17 elements according to the demand quantity; dissolving CuSO4.5H2O in 1L of distilled water for later use; and respectively unsealing and using according to needs. The matrices which can be prepared by the method have characteristics of high precision, convenience in operation, high speed, simple application and small errors between different batches and in the same batch; and furthermore, the phenomena of great errors, precipitation, mildewing and the like caused by influence of manual operation, such as calculation and weighing and external factors, such as weather, temperature and water are avoided, and the method can be applied to plant tissue culture and other biotechnical applications.

The invention discloses a massive preparation method of a high-precision 1/2 MS (Murashige & Skoog) culture substrate, and belongs to the technical field of plant biology. The method comprises the following steps: calculating 1000-times expanded quantity demand of 16 elements contained in the substrate and 10000-times expanded quantity demand of CuSO4.5H2O and CoCl2.6H2O; accurately weighting drug quantity; sequentially mixing the 1000-times expanded 16 elements according to the quantity demand from small to large; sealing; dissolving CuSO4.5H2O and CoCl2.6H2O with 1L of distilled water for later use; respectively opening to use as required. According to the method, the prepared substrate is high in precision; the method has the characteristics of being convenient and fast to operate, simple to apply, and small in error of different batches and the same batch, and is applicable to plant tissue culture and other biological applications.

The invention discloses a large-scale preparation method of a high-precision B5 (containing carbon sources) culture medium, belonging to the plant biotechnology field. The large-scale preparation method comprises the following steps: firstly computing the quantity demands after 16 elements contained in the culture medium are increased by 1000 times and the quantity demands after CuSO4.5H2O and CoCl2.6H2O are increased by 10000 times; accurately weighing chemicals according to the chemical quantities after increase; successively mixing the 16 elements according to the quantity demands and sealing a solid medium; dissolving CuSO4.5H2O and CoCl2.6H2O with 1L of distilled water for later use; and respectively unsealing the solid medium as needed. The large-scale preparation method has the beneficial effects that the high-precision B5 (containing carbon sources) culture medium prepared by the method has the characteristics of convenience and quickness in operation, simplicity in application and small errors between different batches and in the batches; the phenomena such as large errors, precipitation and mouldiness, caused by manual operations and impacts of external factors, are avoided; the method can be applied to the biotechnology such as plant tissue culture.

The invention discloses a scale production method of a high-accuracy MS culture substrate, and belongs to the technical field of plant biology. The method comprises the following steps: firstly, calculating the quantity demand of 18 elements which are contained in the MS basic solid culture substrate and are enlarged by 1,000 times and the quantity demand of CuSO<4>.5H<2>O and CoCl<2>.6H<2>O which is enlarged by 10,000 times; accurately weighing drugs; sequentially mixing and sealing the 16 elements according to the quantity demand; dissolving CuSO<4>.5H<2>O and CoCl<2>.6H<2>O by using 1L of distilled water for later use; unsealing and using respectively according to the requirement. According to the method, the prepared substrate has the characteristics of high accuracy, convenience and quickness in operation, simpleness in application, small errors in different batches and in the same batches; particularly, the weighing errors of the drugs CuSO<4>.5H<2>O and CoCl<2>.6H<2>O with extremely low contents are avoided, and the scale production method can be applied to biological technologies such as plant tissue culture.