Large-scale production method for AA (containing solid medium) solid culture medium
A technology of fixing medium and production method, which is applied in the field of plant biology, and can solve the problems of affecting test results, prone to calculation errors, waste of raw materials, etc.
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[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this example, a large-scale production method of an AA (including fixation medium) solid culture substrate includes five steps of accurate calculation, accurate weighing, first mixing less and then more, sealing, and unsealing for use.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 19 elements contained in the solid basic base of AA (including fixed medium) by 1000 times, that is, calcium chloride dihydrate 440g, potassium dihydrogen phosphate 170g, magnesium sulfate heptahydrate 370g, potassium chloride 2940g, potassium iodide 0.83g, boric acid 6.2g, sodium molybdate dihydrate 0.25g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, cobalt chloride hexahydrate and copper sulfate pentahydrate 0.025g each, sodium iron edetate 36.5g, inositol 100g, thiamine hydrochloride 0.5g, g...
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