Large-scale preparation method for high-precision 1/4MS (containing a fixed medium and a carbon source) culture matrix

Abstract

The invention discloses a large-scale preparation method for a high-precision 1 / 4MS (containing a fixed medium and a carbon source) culture matrix, and belongs to the field of plant biotechnology. The method comprises the following steps: firstly, calculating the demand quantity of 18 elements, amplified by 10,000 times, in the matrix and the demand quantity of CuSO4.5H2O and CoCl2.6H2O amplified by 1,000 times; secondly, accurately weighing amplified drug quantity; thirdly, mixing the 18 elements according to the demand quantity successively and sealing; fourthly, dissolving the demand quantity of CuSO4.5H2O and CoCl2.6H2O in 1 L of distilled water for later use; finally, unsealing for use according to the needs respectively. The matrix prepared with the method has the characteristics of high precision, convenience for operation, quickness, simplicity for application, different batches and small errors in the batches, avoids the phenomena of big errors, deposition, mildewing and the like caused by influence of manual operations and external factors, and can be applied to biotechnologies of plant tissue culture and the like.

Description

technical field [0001] The invention discloses a large-scale production method of a high-precision 1 / 4MS (containing carbon source and fixed medium) culture substrate, belonging to the field of plant biotechnology. More specifically, it includes six steps including accurate calculation, accurate weighing, mixing less and more, sealing, separate mother liquor preparation, unpacking and using, in order to obtain different batches and batches with minimal error A method for cultivating substrates and reducing experimental errors. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic el...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters