Large-scale preparation method for high-precision 1/4MS (containing a fixed medium and a carbon source) culture matrix
A technology for cultivating substrates and fixed media, applied in the field of plant biology, can solve the problems of different colors, instrument precision errors, manufacturers, and quality inconsistencies, and achieve the effect of high precision and small weighing errors.
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[0013] The present invention will be further described below in conjunction with specific embodiments.
[0014] In this embodiment, a high-precision 1 / 4MS (containing carbon source and fixed medium) (containing carbon source and fixed medium and fixed medium) solid culture substrate large-scale production method, including accurate calculation, accurate weighing, first less Afterwards, there are six steps of mixing, sealing, separate mother liquor preparation, and unsealing for use.
[0015] The specific operation of the above six steps is as follows:
[0016] ① Accurate calculation: expand the 18 elements contained in the MS basic medium by 1000 times, that is, 0.1g of thiamine hydrochloride, 0.25g of sodium molybdate dihydrate, 0.5g of nicotinic acid, 0.5g of pyridoxine hydrochloride, and 0.83g of potassium iodide , glycine 2g, boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, sodium iron edetate 36.5g, inositol 100g, potassium dihydroge...
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