Large-scale preparation method of high-precision improved White (containing carbon source and fixed medium) culture medium

Abstract

The invention discloses a large-scale preparation method of a high-precision improved White (containing a carbon source and a fixed medium) culture medium, and belongs to the technical field of plant biology. The method comprises the following steps: firstly, calculating the demanded quantity of 19 elements, which are amplified by 1,000 times, contained in the medium and the demanded quantity of CuSO4.5H2O, which is amplified by 100,000 times; accurately weighing the amplified medicines; successively mixing the 19 elements according to the demanded quantity and sealing; dissolving the CuSO4.5H2O with 1L of distilled water and then storing for later use; and respectively unsealing according to the requirements. The medium prepared by adopting the method has the characteristics of being high in precision, convenient and fast to operate, easy to apply, different in batch and small in errors in batches; the phenomena, such as large errors, participation and mildewing, caused by manual operations and external factors are avoided; and the preparation method can be applied to the biotechnologies such as plant tissue culture.

Description

technical field [0001] The invention discloses a large-scale production method of high-precision improved White (containing carbon source and fixed medium) culture substrate, which belongs to the field of plant biotechnology. More specifically, it includes six steps including accurate calculation, accurate weighing, mixing less and more, sealing, separate mother liquor preparation, unpacking and using, in order to obtain different batches and batches with minimal error A method for cultivating substrates and reducing experimental errors. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters