Large-scale manufacturing method of high-precision C17 culture matrices
A technology for cultivating substrates and production methods, applied in the field of plant biology, which can solve the problems of instrument precision errors, different colors, influences, etc., and achieve the effect of small weighing errors and high precision
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[0013] The present invention will be further described below in conjunction with specific embodiments.
[0014] In this example, a large-scale production method of high-precision C17 (containing carbon source and fixed medium) solid culture substrate, including accurate calculation, accurate weighing, mixing first and then more, sealing, preparation of separate mother liquor, unpacking and using Wait for six steps.
[0015] The specific operation of the above six steps is as follows:
[0016] ① Accurate calculation: expand the 16 elements contained in C17 basic medium by 1000 times, namely niacin 0.5g, thiamine hydrochloride 0.5g, pyridoxine hydrochloride 0.5g, potassium iodide 0.83g, biotin 1.5g, glycine 2g , boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 11.2g, sodium iron edetate 36.5g, inositol 100g, magnesium sulfate heptahydrate 150g, calcium chloride dihydrate 150g, ammonium nitrate 300g, Potassium dihydrogen phosphate 400g, potassium ...
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