Large-scale preparation method for improved Nitsch basic solid culture matrix

Abstract

The invention discloses a large-scale preparation method for an improved Nitsch basic solid culture matrix, and belongs to the field of plant biotechnology. The method comprises the following steps: firstly, calculating the demand quantity of 15 elements, amplified by 1,000 times, in the matrix; secondly, accurately weighing amplified drug quantity, wherein CuSO4.5H2O and Na2MoO4.2H2O need to be accurately weighed by a one ten-thousandth balance; thirdly, mixing the 15 elements according to the demand quantity successively and sealing; finally, unsealing for use according to the needs respectively. The matrix prepared with the method has the characteristics of convenience for operation, quickness, simplicity for application, different batches and small errors in the batches, avoids the phenomena of big errors, deposition, mildewing and the like caused by influence of manual operations of calculating, weighing and the like as well as external factors of weather, temperature, moisture and the like during preparation of a Nitsch liquid culture matrix, and can be applied to biotechnologies of plant tissue culture and the like.

Description

technical field [0001] The invention discloses a large-scale production method of an improved Nitsch basic solid culture substrate, which belongs to the field of plant biotechnology. More specifically, it is a five-step process including accurate calculation, accurate weighing, mixing less and more, sealing, and unsealing, in order to obtain culture substrates with minimal errors between different batches and batches, and reduce Method of trial and error. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different,...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters