Method for preparing MT (mounting medium-and-carbon source containing) solid culture medium in large scale
A technology of fixing medium and production method, applied in the field of plant biology, can solve the problems of precipitation, mother liquor infection of bacteria, mold, and easy calculation errors.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment Construction
[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this embodiment, a large-scale production method of MT (containing carbon source and fixed medium) solid culture substrate includes five steps of accurate calculation, accurate weighing, mixing less and more, sealing, and unsealing for use.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 18 elements contained in the solid base of MT (including carbon source and fixed medium) by 1000 times, that is, 0.025g each of cobalt chloride hexahydrate and copper sulfate pentahydrate, 0.5g of nicotinic acid, and pyridoxine hydrochloride 0.5g, potassium iodide 0.83g, glycine 2g, boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, sodium iron edetate 36.5g, inositol 100g, potassium dihydrogen phosphate 170g, heptahydrate Magnesium sulfate 370g, calcium chloride dihydrate 440g,...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com