Large-scale preparation method of improved Nitsch (containing fixed media and carbon sources) solid culture medium

Abstract

The invention discloses a large-scale preparation method of an improved Nitsch (containing fixed media and carbon sources) solid culture medium, belonging to the plant biotechnology field. The large-scale preparation method comprises the following steps: firstly computing the quantity demands after 17 elements contained in the medium are increased by 1000 times; accurately weighing chemicals according to the chemical quantities after increase, wherein CuSO4.5H2O and Na2MoO4.2H2O need to be accurately weighed with a one over ten-thousand balance; successively mixing the 17 elements according to the quantity demands and sealing a solid culture medium; and respectively unsealing the solid culture medium as needed to be used. The large-scale preparation method has the beneficial effects that the medium prepared by the method has the characteristics of convenience and quickness in operation, simplicity in application and small errors between different batches and in the batches; the phenomena such as large errors, precipitation and mouldiness, caused by manual operations such as computation and weighing, and impacts of external factors such as weather, temperatures and moisture, are avoided; the method can be applied to the biotechnology such as plant tissue culture.

Description

technical field [0001] The invention discloses a large-scale production method of an improved Nitsch (containing fixed medium and carbon source) solid culture substrate, which belongs to the field of plant biotechnology. More specifically, it is a five-step process including accurate calculation, accurate weighing, mixing less and more, sealing, and unsealing, in order to obtain culture substrates with minimal errors between different batches and batches, and reduce Method of trial and error. Background technique [0002] Plant tissue culture technology or soilless culture technology usually requires the preparation of the mother liquid of the medium, that is, the preparation of a liquid that is enlarged by a certain multiple and contains various types of reagents. However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples...

AI Technical Summary

Problems solved by technology

However, because the mother liquor contains different types of reagents, it is often divided into four major categories: macroelements, trace elements, iron salts and organic elements, and the expansion multiples of the four categories are different, resulting in inconsistent quality of medicines, and one is prone to calculation errors; Two, the manufacturer and quality of the medicine are inconsistent, even if the calculation and weighing are consistent, it will affect the test results; three, because the mother liquor is in a liquid state, at the turn of spring and summer, due to the increase in temperature and humidity, it is extremely easy to cause mother liquor infection Bacteria and mold cause different colors or precipitation in the mother liquor, which wastes raw materials; four will cause large errors due to the difference in the scale of the container (especially trace elements) and the precision of the instrument.

[0003] In view of the above-mentioned shortcomings of mother liquor preparation, the preparation of culture medium with conventional mother liquor often leads to failure or unrepeatable test results due to factors such as weighers, drugs, seasons, etc., which greatly affects the use of plant tissue culture or culture media. The process of carrying out trials of soilless cultivation technology has hit the confidence of the experimenters