Large-scale preparation method of high-precision MS (containing carbon sources and fixed media) culture medium
A technology for cultivating substrates and fixed media, applied in the field of plant biology, can solve the problems of instrument precision error, different colors, influence, etc., and achieve the effect of small weighing error and high precision
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[0013] The present invention will be further described below in conjunction with specific embodiments.
[0014] In this embodiment, a large-scale production method of high-precision MS (containing carbon source and fixed medium) (containing carbon source and fixed medium and fixed medium) solid culture substrate, including accurate calculation, accurate weighing, first less and then more There are six steps including ground mixing, sealing, separate mother liquor preparation, unsealing and use.
[0015] The specific operation of the above six steps is as follows:
[0016] ① Accurate calculation: expand the 18 elements contained in the MS basic medium by 1000 times, that is, 0.1g of thiamine hydrochloride, 0.25g of sodium molybdate dihydrate, 0.5g of nicotinic acid, 0.5g of pyridoxine hydrochloride, and 0.83g of potassium iodide , glycine 2g, boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, sodium iron edetate 36.5g, inositol 100g, potassi...
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