Massive preparation method of high-precision MS (Murashige & Skoog) (containing carbon source) culture substrate
A technology for culturing substrates and production methods, applied in the field of plant biology, can solve the problems of instrument precision error, different colors, influence, etc., and achieve the effect of small weighing error and high precision
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[0013] The present invention will be further described below in conjunction with specific embodiments.
[0014] In this embodiment, a large-scale production method of high-precision MS (containing carbon source) (containing carbon source and fixed medium) solid culture substrate includes accurate calculation, accurate weighing, mixing first and then more, sealing, separate Six steps including mother liquor preparation, unpacking and use.
[0015] The specific operation of the above six steps is as follows:
[0016] ① Accurate calculation: expand the 17 elements contained in the MS basic medium by 1000 times, namely 0.1g thiamine hydrochloride, 0.25g sodium molybdate dihydrate, 0.5g nicotinic acid, 0.5g pyridoxine hydrochloride, and 0.83g potassium iodide , glycine 2g, boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, sodium iron edetate 36.5g, inositol 100g, potassium dihydrogen phosphate 170g, magnesium sulfate heptahydrate 370g, dihydrat...
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