Large-scale manufacturing method of C17 (containing carbon source) basic solid culture matrices
A technology of solid culture medium and production method, applied in the field of plant biology, can solve the problems of manufacturers, inconsistent quality, waste of raw materials, influence, etc.
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[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this embodiment, a large-scale production method of C17 (containing carbon source) basic solid culture substrate includes five steps of accurate calculation, accurate weighing, first mixing less and then more, sealing, and unsealing for use.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 18 elements contained in C17 (containing carbon source) basic solid medium by 1000 times, that is, copper sulfate pentahydrate 0.025g, nicotinic acid 0.5g, thiamine hydrochloride 0.5g, pyridoxine hydrochloride 0.5 g, potassium iodide 0.83g, biotin 1.5g, glycine 2g, boric acid 6.2g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 11.2g, sodium iron edetate 36.5g, inositol 100g, magnesium sulfate heptahydrate 150g, 150g calcium chloride dihydrate, 300g ammonium nitrate, 400g potassium dihydrogen phos...
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