Large-scale manufacturing method of AA (containing carbon source and fixed medium) solid culture matrices
A technology of fixing medium and production method, applied in the field of plant biology, can solve the problems of different colors, effects, precipitation, etc.
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[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this embodiment, a large-scale production method of an AA (containing carbon source and fixed medium) solid culture substrate includes five steps of accurate calculation, accurate weighing, first mixing less and then more, sealing, and unsealing for use.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 20 elements contained in the solid basic base of AA (including carbon source and fixed medium) by 1000 times, that is, calcium chloride dihydrate 440g, potassium dihydrogen phosphate 170g, magnesium sulfate heptahydrate 370g, potassium chloride 2940g, potassium iodide 0.83g, boric acid 6.2g, sodium molybdate dihydrate 0.25g, zinc sulfate heptahydrate 8.6g, manganese sulfate tetrahydrate 22.3g, cobalt chloride hexahydrate and copper sulfate pentahydrate each 0.025g, ethylenediamine tetrahydrate Sodium...
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