Large-scale preparation method of 1/4 MS (containing carbon sources) basic solid culture matrix
A solid culture and production method technology, applied in the field of plant biology, can solve the problems of manufacturers, inconsistent quality, waste of raw materials, and impact
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[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this embodiment, a large-scale production method of 1 / 4MS (containing carbon source) basic solid culture substrate includes five steps of accurate calculation, accurate weighing, first mixing less and then more, sealing, and unsealing for use.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 19 elements contained in the basic solid base of 1 / 4MS (including fixed medium) by 1000 times, that is, 0.025g each of cobalt chloride hexahydrate and copper sulfate pentahydrate, 0.1g of thiamine hydrochloride, and 0.1g of dihydrate Sodium molybdate 0.25g, Nicotinic acid 0.5g, Pyridoxine hydrochloride 0.5g, Potassium iodide 0.83g, Glycine 2g, Boric acid 6.2g, Zinc sulfate heptahydrate 8.6g, Manganese sulfate tetrahydrate 22.3g, Sodium iron edetate 36.5g, potassium dihydrogen phosphate 42.5g, magnesium sulfate ...
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