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Melanoma cell adhesion molecule antagonist polypeptide and application thereof

A technology of melanoma cells and adhesion molecules, which is applied in the field of melanoma cell adhesion molecule antagonist polypeptides, can solve the problems of melanoma cell adhesion molecule antagonist polypeptides, etc., and achieve the effect of improving survival rate and good curative effect

Inactive Publication Date: 2015-06-24
SUZHOU PULUODA BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no well-developed melanoma cell adhesion molecule antagonist polypeptide available for the treatment of malignant tumors

Method used

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  • Melanoma cell adhesion molecule antagonist polypeptide and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Effect of melanoma cell adhesion molecule antagonist polypeptide on migration of human vascular endothelial cells.

[0027] Using scratch test. First use a marker pen on the back of the 24-well plate, draw a horizontal line evenly with a straightedge, about every 0.5-1cm, across the hole. Pass 3 lines per well; divide logarithmically grown HUVEC cells at 1.0×10 5 Added to 24-well culture plate, cultivated for 24h. On the second day, compare the ruler with a 10 μl pipette tip, scratch perpendicular to the horizontal line on the back, and use the intersection of the scratch and the horizontal line on the back as a fixed observation point; add different concentrations of experimental wells and positive drug control wells respectively. The drug melanoma cell adhesion molecule antagonist polypeptide (Shanghai Sangong Synthetic) and the positive control drug vincristine; the same volume of solvent was added to the blank group, and five duplicate holes were set in each well;...

Embodiment 2

[0029] IC50 of melanoma cell adhesion molecule antagonist polypeptide on the growth and survival of human vascular endothelial cells cultured in vitro.

[0030] The MTT colorimetric method was used. The logarithmic growth of human vascular endothelial cells HUVEC, 1.0 × 10 5 Add it into a 96-well culture plate, culture for 24 hours, add different concentrations of the experimental drug melanoma cell adhesion molecule antagonist polypeptide (Shanghai Sangong Synthetic) and the positive control drug paclitaxel to the experimental wells and positive drug control wells; add the same volume to the blank group solvent. Set up five duplicate wells per well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h of action, incubate for 30min, and place at 620nm in a microplate reader Measure the absorbance A value, according to the formula HUVEC growth inhibition rate=(1-absorbance value of the experimental group / absorbance v...

Embodiment 3

[0032] The in vivo activity of melanoma cell adhesion molecule antagonist polypeptide was detected by tumor model.

[0033] The melanoma tumor model was established, and the positive control drug paclitaxel was added; the same volume of solvent was added to the blank group, and three doses of polypeptide (Shanghai Sangong Synthetic) were set in the experimental group: 0.75, 1.5, and 3 mg / Kg. After 21 days, the number of surviving mice was observed, and the survival rate was calculated. The results show that the melanoma cell adhesion molecule antagonist polypeptide can effectively protect mice and improve the survival rate of tumor-bearing mice, with the survival rate reaching 87.46%.

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Abstract

The invention discloses a melanoma cell adhesion molecule antagonist polypeptide and application thereof, relates to the field of drugs and particularly relates to a polypeptide for inhibiting tumor angiogenesis and treating malignant tumors. The polypeptide has the sequence as follows: VAGVPELGLDVELEVTLLSEP which is a brand new sequence; and the polypeptide can be used for specially inhibiting the propagation and migration of vascular endothelial cells of tumor tissues in vitro, inhibiting the generation of the tumor vessels and increasing the survival rate of tumor-bearing mice in in-vivo experiments, has a potential novel drug development value and can also be used as a kit for detecting tumors.

Description

Technical field [0001] The present invention involves melanoma cell adhesion molecular antagonist polypeptides and its application. It specificly involves specific inhibitory of the proliferation and migration of vascular endothelial cells that inhibit tumor tissue, inhibit tumor blood vessel production, and treat malignant tumor polypeptides. Background technique [0002] In 1971, Folkman proposed that tumor growth and metastasis depended on the generation of new blood vessels.It is believed that after the formation of physical tumors, its development can be divided into two stages: vascular non -vascular and vascular stage.In non -vascular stage, the existence of tiny tumors depends on the surrounding interval to scattered blood supply. The growth rate is linear. The tumor is limited to 1mm to 2mm. 5 ~ 10 6 This is forced to be in a "dormant state"; after entering the blood vessel stage, the tumor tissue is perfusion blood supply, and the tumor body grows indexed, which can inc...

Claims

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Application Information

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IPC IPC(8): C07K14/00A61K38/16A61P35/00G01N33/68G01N33/574
Inventor 罗瑞雪
Owner SUZHOU PULUODA BIOLOGICAL SCI & TECH
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