Culture method for improving yield of botryococcus polysaccharides

A technology of grape algae polysaccharides and culture methods, applied in the field of grape algae cultivation, can solve problems such as the depletion of terrestrial microbial resources, achieve high mutagenesis efficiency and effect, promote cell growth, and high efficiency

Inactive Publication Date: 2015-06-24
CHINA JILIANG UNIV
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  • Abstract
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Problems solved by technology

[0004] On the other hand, most of the widely used antibiotics come from terrestrial actino

Method used

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  • Culture method for improving yield of botryococcus polysaccharides
  • Culture method for improving yield of botryococcus polysaccharides
  • Culture method for improving yield of botryococcus polysaccharides

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Embodiment 1

[0036] (1) Research on the culture technology of grape algae with high polysaccharide yield

[0037] 1 Materials and methods

[0038] 1.1 Experimental materials

[0039] Botrytis was provided by Hangzhou Huadan Agricultural Products Co., Ltd.;

[0040] Penicillin and streptomycin solution: containing penicillin 10000U / ml, streptomycin 10000U / ml;

[0041] VS-840K-U ultra-clean bench, Suzhou Antai Air Technology Co., Ltd.;

[0042] 722S visible spectrophotometer, Shanghai Prism Technology Co., Ltd.;

[0043] TGL-16G desktop high-speed centrifuge, Hunan Xingke Scientific Instrument Co., Ltd.;

[0044] ZHWY-2102C constant temperature shaker incubator, Shanghai Zhicheng Analytical Instrument Manufacturing Co., Ltd.;

[0045] HH-2 digital display constant temperature water bath, Shanghai Changsi Industry and Trade Co., Ltd.;

[0046] PGX multi-segment light incubator, Ningbo Laifu Technology Co., Ltd.;

[0047] Argon ion laser, Coherent Innova 308C, USA;

[0048] CY-5D Ultra...

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Abstract

The invention discloses a culture method for improving the yield of botryococcus polysaccharides, comprising the following steps: culturing purified botryococcus to a logarithmic growth period, selecting botryococcus liquid in logarithmic growth, performing induced mutation under the irradiation of argon ion laser, then inoculating to a BG11 liquid medium, and culturing under the conditions that the temperature is 28-33 DEG C, the illumination intensity is 43-86mumol.m<-2>.s<-1> and the illumination period is 14 hours every day; performing ultrasonic radiation treatment after culturing 2-3 days, continuously culturing the botryococcus subjected to the ultrasonic radiation treatment for 12-13 days under the conditions that the culture temperature is 28-33 DEG C, the illumination intensity is 43-86mumol.m<-2>.s<-1> and the illumination period is 14 hours every day, and performing centrifugal separation and sterile water washing on the obtained culture solution to obtain botryococcus rich in polysaccharides. By combining the argon ion laser induced mutation with the ultrasonic treatment, the yield of the cultured botryococcus is high, many polysaccharides are accumulated, and the polysaccharide content of the botryococcus can reach 35.99% of the dry weight.

Description

(1) Technical field [0001] The invention relates to a method for cultivating grape algae with antibacterial effect and high polysaccharide production. (2) Background technology [0002] Environmental safety, aquaculture safety and other issues brought about by the over-exploitation of the fishery and the rapid development of the aquaculture industry have become more and more prominent, and the long-term abuse of drugs has resulted in increased bacterial resistance, drug residues, and environmental pollution. Outstanding issue. At the same time, with the development of industrialized high-density farming, a large amount of artificial feed is used, which dissolves a large amount of bait residues and excrement of aquatic animals in the water, causing serious pollution of the breeding water and causing serious damage to the growth and development of the farmed animals. At present, the substitutes of feed antibiotics on the market are mainly probiotics or traditional herbal medi...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12N13/00C12N1/12C12R1/89
Inventor 张拥军苏东洋王微董铮朱丽云
Owner CHINA JILIANG UNIV
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