Quality detection method for qi-moving Tannikaer capsule
A quality inspection method and gas-activating tennecal technology, applied in the field of chemical analysis, can solve the problems of cumbersome operation, long time consumption, unfavorable environmental protection, etc., and achieve the effect of ensuring drug safety.
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Embodiment 1
[0186] Example 1 Preparation of Xingqi Tennical Capsules
[0187] API prescription:
[0188] Aloe Vera 230.4g, Pepper 172.8g, Hyacinth 28.8g, Borax 36.0g.
[0189] Prepared by:
[0190] The above four flavors are crushed into fine powder, sieved, mixed evenly, packed into capsules, 0.3g per capsule, 1000 capsules in total.
Embodiment 2
[0191] Example 2 Quantitative determination of aloin and piperine by high performance liquid chromatography
[0192] Determined with reference to the high-performance liquid phase method recorded in Appendix VID of the Chinese Pharmacopoeia 2010 edition.
[0193] High-performance liquid chromatography: Agilent 1100; data processor: Agilent 1100 ChemStation; ultraviolet detector: VWD G1314A.
[0194] Chromatographic conditions and system suitability
[0195] Column: AKZONOBEL Kromasil 100-5C 18 , 4.6×250mm;
[0196] Column temperature: 30°C;
[0197] Mobile phase: Acetonitrile was used as mobile phase A, water was used as mobile phase B, gradient elution, and the elution program was shown in Table 1;
[0198] Flow rate: 1.0ml / min;
[0199] Detection wavelength: 350nm;
[0200] The number of theoretical plates calculated based on the aloin peak should not be less than 5000.
[0201] Preparation of Reference Substance Solution Take an appropriate amount of aloin reference ...
Embodiment 3
[0205] Example 3 TLC Qualitative Identification of Borax
[0206] Preparation of the test solution: take 1.5 g of the contents of the three batches of capsules prepared according to Example 1, put them in a crucible, heat slowly until completely charred, let cool to room temperature, place the carbonized matter in a mortar, grind finely, transfer To a centrifuge test tube, add 10ml of water, power 250W, ultrasonic treatment at a frequency of 35kHz for 10min, centrifuge at a speed of 6000 rpm for 10min, filter, concentrate the filtrate to dryness, add 1ml of methanol to the residue to dissolve, add 1 drop of sulfuric acid, and let stand. Get supernatant as need testing solution;
[0207] The Xingqi Tannecal Capsules with batch numbers 20111001, 20111002 and 20111003 were prepared respectively according to the above-mentioned method.
[0208] Preparation of reference solution: Take another 50 mg of borax, add 2 ml of methanol, sonicate to dissolve, add 1 drop of sulfuric acid...
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