Refined polyphenol with tumor suppression function as well as preparation method and application of polyphenol

Abstract

The invention discloses a refined polyphenol which is prepared from Trapa acornis Nakano shells and has a tumor suppression function as well as a preparation method and an application of the polyphenol. The method comprises steps as follows: 1) the Trapa acornis Nakano shells are dried in the shade, crushed and sieved; 2) a crude extract is prepared; 3) the refined polyphenol is prepared. The refined polyphenol has a very good inhibition effect on A549, MDA-MB-231, MCF-7 and LOVO tumor cells in in-vitro cell experiments. The waste Trapa acornis Nakano shells are used as a raw material, active components are prepared with a modern extraction and separation means and pharmacological activity screening combined method, waste is turned into wealth, and an anti-tumor drug is expected to be developed.

Description

Technical field

[0001] The present invention relates to a refined polyphenol of water chestnut shell and a preparation method and application thereof, in particular to a refined polyphenol of Nanhu water chestnut shell with tumor inhibitory effect, a preparation method and application thereof, and belongs to the technical field of plant natural products. Background technique

[0002] Plant polyphenols, also called tannins and tannins, are a general term for a class of secondary metabolites that are widely present in plants and have multiple phenolic hydroxyl groups in their molecules. Regarding its medicinal value, such as anti-oxidation, antibacterial, anti-aging, anti-tumor, anti-virus, and its therapeutic effects on cardiovascular and cerebrovascular diseases and cancer, it has always been a hot spot for researchers at home and abroad. Trapa acornis Nakano (Trapa acornis Nakano), also known as Qingling, Wontonling, and Yuanbaoling, is an annual aquatic herb of Trapaaceae in th...

Examples

Embodiment 1

[0018] A preparation method of refined polyphenol of Nanhu water chestnut, the steps are as follows:

[0019] (1) Pretreatment of water chestnut shell: Peel off the fruit of Nanhu water chestnut, leave the shell, dry it in a ventilated and cool place, then crush it with a plant grinder and pass through a 60-mesh sieve. Collect the powder as the raw material and place it in a cool and dry place. spare.

[0020] (2) Preparation of crude extract: Add the raw material obtained in step 1) to a 50% ethanol solution at a material-to-liquid ratio of 1:20 (g / ml), then flash extraction at room temperature for 20 seconds, filter and collect the filtrate Centrifuge at 3000r / min for 10min, collect the supernatant, and concentrate under reduced pressure to obtain a crude extract.

[0021] (3) Preparation of refined polyphenols: dissolve the polyphenol extract obtained in step 2) with a small amount of water and directly enter the D101 macroporous adsorption resin column, first rinse with 2 column...

Embodiment 2

[0023] A preparation method of refined polyphenol of Nanhu water chestnut, the steps are as follows:

[0024] (1) Pretreatment of water chestnut shell: Peel off the fruit of Nanhu water chestnut, leave the shell, dry it in a ventilated and cool place, then crush it with a plant grinder and pass through a 60 mesh sieve. Collect the powder as the raw material and place it in a cool and dry place. spare.

[0025] (2) Preparation of crude extract: Add the raw materials obtained in step 1) to a 50% ethanol solution at a material-to-liquid ratio of 1:30 (g / ml), then flash extraction at room temperature for 30s, filter and collect the filtrate , The filter residue was extracted twice again, the filtrate was combined, centrifuged at 3000r / min for 10min, the supernatant was collected, and concentrated under reduced pressure to obtain a crude extract.

[0026] (3) Preparation of refined polyphenols: dissolve the polyphenol extract obtained in step 2) with a small amount of water and directly ...

Embodiment 3

[0028] A preparation method of refined polyphenol of Nanhu water chestnut, the steps are as follows:

[0029] (1) Pretreatment of water chestnut shell: Peel off the fruit of Nanhu water chestnut, leave the shell, dry it in a ventilated and cool place, then crush it with a plant grinder and pass through a 60-mesh sieve. Collect the powder as the raw material and place it in a cool and dry place. spare.

[0030] (2) Preparation of crude extract: Add the raw materials obtained in step 1) to a 70% ethanol solution according to a material-to-liquid ratio of 1:30 (g / ml), then flash extraction at room temperature for 40s, filter and collect the filtrate , The filter residue was extracted twice again, the filtrate was combined, centrifuged at 3000r / min for 10min, the supernatant was collected, and concentrated under reduced pressure to obtain a crude extract.

[0031] (3) Preparation of refined polyphenols: dissolve the polyphenol extract obtained in step 2) with a small amount of water and...

Technical field

[0001] The present invention relates to a refined polyphenol of water chestnut shell and a preparation method and application thereof, in particular to a refined polyphenol of Nanhu water chestnut shell with tumor inhibitory effect, a preparation method and application thereof, and belongs to the technical field of plant natural products. Background technique

[0002] Plant polyphenols, also called tannins and tannins, are a general term for a class of secondary metabolites that are widely present in plants and have multiple phenolic hydroxyl groups in their molecules. Regarding its medicinal value, such as anti-oxidation, antibacterial, anti-aging, anti-tumor, anti-virus, and its therapeutic effects on cardiovascular and cerebrovascular diseases and cancer, it has always been a hot spot for researchers at home and abroad. Trapa acornis Nakano (Trapa acornis Nakano), also known as Qingling, Wontonling, and Yuanbaoling, is an annual aquatic herb of Trapaaceae in th...

Examples

Embodiment 1

[0018] A preparation method of refined polyphenol of Nanhu water chestnut, the steps are as follows:

[0019] (1) Pretreatment of water chestnut shell: Peel off the fruit of Nanhu water chestnut, leave the shell, dry it in a ventilated and cool place, then crush it with a plant grinder and pass through a 60-mesh sieve. Collect the powder as the raw material and place it in a cool and dry place. spare.

[0020] (2) Preparation of crude extract: Add the raw material obtained in step 1) to a 50% ethanol solution at a material-to-liquid ratio of 1:20 (g / ml), then flash extraction at room temperature for 20 seconds, filter and collect the filtrate Centrifuge at 3000r / min for 10min, collect the supernatant, and concentrate under reduced pressure to obtain a crude extract.

[0021] (3) Preparation of refined polyphenols: dissolve the polyphenol extract obtained in step 2) with a small amount of water and directly enter the D101 macroporous adsorption resin column, first rinse with 2 column...

Embodiment 2

[0023] A preparation method of refined polyphenol of Nanhu water chestnut, the steps are as follows:

[0024] (1) Pretreatment of water chestnut shell: Peel off the fruit of Nanhu water chestnut, leave the shell, dry it in a ventilated and cool place, then crush it with a plant grinder and pass through a 60 mesh sieve. Collect the powder as the raw material and place it in a cool and dry place. spare.

[0025] (2) Preparation of crude extract: Add the raw materials obtained in step 1) to a 50% ethanol solution at a material-to-liquid ratio of 1:30 (g / ml), then flash extraction at room temperature for 30s, filter and collect the filtrate , The filter residue was extracted twice again, the filtrate was combined, centrifuged at 3000r / min for 10min, the supernatant was collected, and concentrated under reduced pressure to obtain a crude extract.

[0026] (3) Preparation of refined polyphenols: dissolve the polyphenol extract obtained in step 2) with a small amount of water and directly ...

Embodiment 3

[0028] A preparation method of refined polyphenol of Nanhu water chestnut, the steps are as follows:

[0029] (1) Pretreatment of water chestnut shell: Peel off the fruit of Nanhu water chestnut, leave the shell, dry it in a ventilated and cool place, then crush it with a plant grinder and pass through a 60-mesh sieve. Collect the powder as the raw material and place it in a cool and dry place. spare.

[0030] (2) Preparation of crude extract: Add the raw materials obtained in step 1) to a 70% ethanol solution according to a material-to-liquid ratio of 1:30 (g / ml), then flash extraction at room temperature for 40s, filter and collect the filtrate , The filter residue was extracted twice again, the filtrate was combined, centrifuged at 3000r / min for 10min, the supernatant was collected, and concentrated under reduced pressure to obtain a crude extract.

[0031] (3) Preparation of refined polyphenols: dissolve the polyphenol extract obtained in step 2) with a small amount of water and...

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