Propagation method of dendrobe seeds

Abstract

The invention discloses a propagation method of dendrobe seeds, which belongs to the technical field of artificial cultivation of dendrobe. The method comprises the following steps of: seeding in a seeding medium, performing propagation cultivation in a propagation medium, performing rooting cultivation in a rooting medium, and performing strong seedling cultivation in a strong seedling medium, so as to obtain cultivation seedlings. According to the propagation method, the technical problems of small base of explants, difficulty in disinfection, slowness in differentiation of original corm, long propagation cycle, low propagation multiple, difficulty in establishment of an asepsis system and the like of the prior art are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of dendrobium, in particular to a seed propagation method of dendrobium. Background technique [0002] In the production of Dendrobium seedlings, the fruits (seeds) are generally used as explants for rapid propagation. However, the seedlings bred by this method have limited traits of the mother body, large differences in traits, miscellaneous varieties, large variations, easy degradation, difficult quality assurance, and difficult maintenance of excellent seed properties. And when using stem tip or stem segment as propagation material, when carrying out tissue culture asexual rapid propagation, although can obtain the seedling with strong target selection, consistent character, consistent quality, high purity, but there is explant base number is small, difficult to sterilize , slow differentiation of protocorms, long propagation cycle, low multiplication factor, difficult establish...

AI Technical Summary

Problems solved by technology

However, for the seedlings bred by this method, the traits of the mother body are limited, the traits are greatly different, the varieties are mixed, the variation is large, it is easy to degenerate, the quality is difficult to guarantee, and it is difficult to maintain good seed properties.

And when using stem tip or stem segment as propagation material, when carrying out tissue culture asexual rapid propagation, although can obtain the seedling with strong target selection, consistent character, consistent quality, high purity, but there is explant base number is small, is not easy to sterilize , slow differentiation of protocorms, long reproductive cycle, low multiplication factor, difficult establishment of sterile system and other technical problems, so it is not widely used in actual production