Propagation method of dendrobe seeds

A technology for seeds and Dendrobium, which is applied in the field of Dendrobium seed reproduction, can solve the problems of difficulty in maintaining excellent species, long reproduction cycle, and miscellaneous varieties.

Inactive Publication Date: 2015-07-29
四川省峰上生物科技有限公司
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  • Abstract
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Problems solved by technology

However, for the seedlings bred by this method, the traits of the mother body are limited, the traits are greatly different, the varieties are mixed, the variation is large, it is easy to degenerate, the quality is difficult to guarantee, and it is difficult to maintain good seed properties.
And when using stem tip or stem segment as propagation material, when carrying out tissue culture asexual rapid propagation, although can obtain the seedling with strong target selection, consistent character, consistent quality, high purity, but there is explant base number is small, is not easy to sterilize , slow differentiation of protocorms, long reproductive cycle, low multiplication factor, difficult establishment of sterile system and other technical problems, so it is not widely used in actual production

Method used

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Embodiment Construction

[0014] The present invention will be further described in detail below in conjunction with specific embodiments.

[0015] A kind of dendrobium seed propagation method is characterized in that: comprises the following steps:

[0016] (1) Sowing: In a sterile environment, the seeds were placed in the sowing medium, the pH value of the medium was 5.8, the culture temperature was 25-27°C, the light intensity was 2300LUX, the light time was 12h, and the culture days were 45 days, and the seeds germinated form protocorms. The ratio of the seeding medium is: potassium nitrate 68g, ammonium sulfate 5.6g, potassium dihydrogen phosphate 12g, magnesium sulfate 16g, calcium chloride 15g, disodium edetate 1.68g, ferrous sulfate 3g, manganese sulfate 2.12 g, zinc sulfate 1.72g, boric acid 2600mg, iodide 20mg, potassium 30mg, vitamin B 1 10mg, niacin 200mg, glycine 8mg, sucrose 20mg, potato 10g, sugar 3g.

[0017] (2) Proliferation: transfer the protocorm to the proliferation medium for p...

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Abstract

The invention discloses a propagation method of dendrobe seeds, which belongs to the technical field of artificial cultivation of dendrobe. The method comprises the following steps of: seeding in a seeding medium, performing propagation cultivation in a propagation medium, performing rooting cultivation in a rooting medium, and performing strong seedling cultivation in a strong seedling medium, so as to obtain cultivation seedlings. According to the propagation method, the technical problems of small base of explants, difficulty in disinfection, slowness in differentiation of original corm, long propagation cycle, low propagation multiple, difficulty in establishment of an asepsis system and the like of the prior art are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation of dendrobium, in particular to a seed propagation method of dendrobium. Background technique [0002] In the production of Dendrobium seedlings, the fruits (seeds) are generally used as explants for rapid propagation. However, the seedlings bred by this method have limited traits of the mother body, large differences in traits, miscellaneous varieties, large variations, easy degradation, difficult quality assurance, and difficult maintenance of excellent seed properties. And when using stem tip or stem segment as propagation material, when carrying out tissue culture asexual rapid propagation, although can obtain the seedling with strong target selection, consistent character, consistent quality, high purity, but there is explant base number is small, difficult to sterilize , slow differentiation of protocorms, long propagation cycle, low multiplication factor, difficult establish...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00A01G1/00
Inventor 汪威锋
Owner 四川省峰上生物科技有限公司
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