Depsipeptide compound, and preparation method and application thereof

A technology for depsipeptides and compounds, which is applied in the field of depsipeptides and their preparation, can solve problems such as restricting the export of agricultural products, and achieve the effects of short cycle, simple production process and good insecticidal activity.

Active Publication Date: 2015-07-29
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, the "green barrier" caused by chemical pesticide residues has seriously restricted the export of agri

Method used

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  • Depsipeptide compound, and preparation method and application thereof
  • Depsipeptide compound, and preparation method and application thereof
  • Depsipeptide compound, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: The depsipeptide compound is shown in formula (1) (the Arabic numerals and Greek letters in the structural formula are the carbon atoms in the chemical structure).

[0023]

Embodiment 2

[0024] Example 2: Fermentative production and separation and purification of depsipeptide compounds:

[0025] 1) Fermentation culture

[0026] Strain culture: According to the conventional culture method of microorganisms, a small amount of Beauveria felina AS-70 strains preserved in agar-malt extract medium was picked, inoculated on the surface of PDA plates, and cultured at 28°C for 3 days as a scale. The strains cultured by fermentation are ready for use.

[0027] An appropriate amount of bacteria on the surface of the above-mentioned PDA plate was cut, inoculated into a sterilized Erlenmeyer flask filled with rice culture medium, and cultured at room temperature for 35 days. Add ethyl acetate to sterilize and set aside.

[0028] The rice culture medium is rice 100g / bottle, peptone 0.6g / bottle, natural sea water 100mL / bottle, and 1000mL Erlenmeyer Erlenmeyer flask.

[0029] 2) Separation and purification of compounds

[0030] The above-mentioned rice culture medium was ...

Embodiment 3

[0035] Embodiment 3: insecticidal activity test

[0036] Artemia (brine shrimp), also known as saltwater Artemia, belongs to the phylum Arthropoda, Crustacea, Order Anocaria, Saltwater Artemia family, and the genus Artemia. As a model for insecticide screening, Artemia has been reported at home and abroad. Wang Zaiqiang et al. [Pesticides, 2011, 50(4): 261–263] used Artemia as a model organism to evaluate the killing effect of 14 common insecticides. Insecticidal activity, the results show that the method of screening compounds with insecticidal activity with Artemia is simple and sensitive to a variety of insecticides with different mechanisms of action; Hu Zhiyu et al. Artemia as an indicator organism, rapid screening of compounds with insecticidal activity in marine actinomycetes; Blizzard T.A. et al [J.Antibiot,1989,42(8):1304–1307] rapid screening of insecticide Avermella with Artemia Analogues of the element. In conclusion, as a model organism for the determination of ...

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Abstract

The invention relates to a microbe-derived pesticide, and concretely relates to a depsipeptide compound, and a preparation method and an application thereof. The depsipeptide compound is represented by formula (1). The depsipeptide compound is obtained by through fermenting culture of Beauveria felina AS-70 (preserved in China General Microbiological Culture Collection Center on Sep. 29, 2012 with the preservation number of CGMCC NO.6643), and through extraction and separation. A result of identification of the chemical structure of the compound through nuclear magnetic resonance and mass spectrum shows that the compound has good insecticidal activity.

Description

technical field [0001] The invention relates to a microbial source insecticide, in particular to a depsipeptide compound and its preparation method and application. Background technique [0002] With the extensive use of chemical pesticides, the resistance of plant diseases and insect pests to them has become more and more obvious, which has also brought problems such as environmental pollution and human and animal poisoning. In recent years, the "green barrier" caused by chemical pesticide residues has seriously restricted the export of agricultural products in my country, and the traditional agricultural industry is also facing greater risks and challenges. Compared with traditional chemically synthesized pesticides, microbial-derived pesticides have the advantages of being safe for humans, animals and non-target organisms, good environmental compatibility, less likely to develop resistance, and easy for large-scale production. Therefore, the development and application o...

Claims

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Application Information

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IPC IPC(8): C07K11/02C12P21/02C07K1/20C07K1/16A01N43/72A01P7/00C12R1/645
Inventor 王斌贵杜丰玉
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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