Method for preparation of high light absorption ratio capsorubin

A technology of capsanthin and capsanthin, applied in chemical instruments and methods, separation/purification of carbonyl compounds, azo dyes, etc., to achieve the effects of fewer solvent types, improved purity and absorbance ratio, and reduced costs

Active Publication Date: 2015-08-12
河北东之星生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary capsanthin (absorbance ratio lower than 1.000) has gradually failed to meet people's needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0026] Capsanthin mainly includes red component and yellow component, and red component includes capsanthin and capsanthin. The present invention separates and prepares capsanthin with high light absorption ratio from capsanthin by column chromatography, and the preparation method comprises the following steps:

[0027] A column packing: silica gel and n-hexane with a mass ratio of 1:1 to 1:4 are stirred evenly and then added to the chromatography column at a constant speed for column packing. The specifications of the chromatography columns used are different, and the amount of silica gel used is different. The diameter of the chromatography column is 30cm, the height of the column is 1m, and the quality of the silica gel is 13-16kg. Use 50kg of n-hexane to compact the column, and reserve a certain liquid level on the solid column to prevent impact on the solid column during subsequent sample loading and affect the adsorption effect;

[0028] B sample loading: Dissolve...

Embodiment 1

[0035] The method for preparing capsanthin with high absorbance ratio of the present embodiment specifically comprises the following steps:

[0036] A column packing: silica gel and n-hexane with a mass ratio of 1:2 are stirred evenly and then added to the chromatography column at a constant speed for column packing. The column diameter of the chromatography column used is 30cm, the column height is 1m, and the quality of silica gel is 13kg. After filling evenly to form a solid column, apply a pressure of 0.1MPa on the basis of normal pressure, add 45kg of n-hexane to the column to compact the column, and leave a certain reserved liquid level on the column;

[0037] B sample loading: the mixed sample after the mass ratio of 1:2 capsanthin and n-hexane is completely dissolved is added to the chromatographic column at a flow rate of 4L / h, the mass ratio of the capsanthin used to the silica gel used when packing the column is 3:10;

[0038] C elution: The yellow componen...

Embodiment 2

[0042] The method for preparing capsanthin with high absorbance ratio of the present embodiment specifically comprises the following steps:

[0043] A column packing: silica gel and n-hexane with a mass ratio of 1:1 are stirred evenly and then added to the chromatography column at a constant speed for column packing. The column diameter of the chromatography column used is 30cm, the column height is 1m, and the quality of silica gel is 15kg. After filling evenly to form a solid column, apply a pressure of 0.07MPa on the basis of normal pressure, add 48kg of n-hexane to the column to compact the column, and leave a certain reserved liquid level on the column;

[0044] B sample loading: the mixed sample after the mass ratio of 1:3 capsanthin and n-hexane is completely dissolved is added to the chromatography column at a flow rate of 3L / h, the mass ratio of the capsanthin used to the silica gel used when packing the column is 4:10;

[0045] C elution: The yellow componen...

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PUM

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Abstract

The invention discloses a method for preparation of high light absorption ratio capsorubin, capsanthin in capsicum red pigment is isolated and prepared by column chromatography, the preparation method comprises the following steps: A packing a column, to be more specific, silica gel and n-hexane are mixed evenly and packed into a chromatography column in uniform velocity; B loading a sample, to be more specific, a mixed sample which is completely dissolved with capsanthin and n-hexane is loaded into the chromatography column; C eluting, to be more specific, a yellow component and the capsanthin in the capsicum red pigment are eluted by gradient elution, and in the elution process, a yellow component eluent and a capsanthin eluent are respectively were collected; and D vacuum concentration, to be more specific, the capsanthin eluent is decompressed and concentrated to obtain the capsanthin. The preparation method is simple in operation, equipment are basic equipment and low in cost, regents are high in safety, and can be used in mass production, and the capsanthin prepared by the method is high in purity, high in light absorption ratio and stable in properties.

Description

technical field [0001] The invention belongs to the technical field of bioseparation, and in particular relates to a method for preparing capsanthin with high light absorption ratio. Background technique [0002] Capsanthin, also known as capsanthin and capsicum oleoresin, is a natural colorant extracted from peppers. The main coloring substance of peppers is capsanthin, which belongs to carotenoid pigments, mainly including polar The red component and the less polar yellow component, the red component is capsanthin and capsanthin, and the main components of the yellow component are carotene and zeaxanthin. Among them, the main coloring components of capsanthin are capsanthin and capsanthin. Capsicum red pigment is bright in color and has remarkable effects in coloring strength and color retention. It can not only be used for safe coloring of various foods, but also has certain nutritional and health effects. [0003] The absorbance ratio of capsanthin refers to the absorb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54C07C49/573C07C45/78
CPCC09B61/00C09B67/0096
Inventor 田瑞玲王红改刘社军王卫东李增友郭改英何志芳
Owner 河北东之星生物科技股份有限公司
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