Preparation method and application of plasma chiral nano-gold dimer based on polarization effect

A polarization effect and dimer technology, applied in the field of material chemistry, can solve the problems of limited treatment effect of target cells and normal tissue damage

Active Publication Date: 2015-08-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current clinical methods for cancer treatment are mainly chemotherapy and surgical treatment. These treatments not only have very limited therapeutic effect on target cells, but also cause serious damage to other normal tissues.

Method used

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  • Preparation method and application of plasma chiral nano-gold dimer based on polarization effect
  • Preparation method and application of plasma chiral nano-gold dimer based on polarization effect
  • Preparation method and application of plasma chiral nano-gold dimer based on polarization effect

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Experimental program
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Effect test

Embodiment 1

[0023] All glass instruments are soaked in aqua regia for 24 hours, washed with double distilled water, and dried for later use. The water used in the experiment was all 18.2 MΩ Milli-Q ultrapure water.

[0024] (1) Surface modification of chiral gold nanoparticles: Take 50mL of 5nM gold nanoparticles (20nm) that have been assembled and centrifuge at 8000rpm for 10min, and resuspend in 5mL 0.01M phosphate buffered saline PBS pH7.4 . Press NH 2 -PEG-SH:PEG-SH:Au has a molar concentration ratio of 1000:100:1 while adding NH to the 5mL resuspended nano-gold dimer sol 2 -PEG-SH and PEG-SH; after shaking at room temperature for 12 hours, centrifuge at 8000 rpm for 10 min, remove the supernatant, and resuspend the pellet in a pH 7.4 PBS solution. Repeated centrifugation and resuspension 3 times to obtain nano gold surface modified with amino groups Au dimer-NH 2 ;

[0025] (2) Coupling of chiral gold nanometer dimer and photosensitive molecule: the gold nanometer dimer Au dimer-NH whos...

Embodiment 2

[0027] Example 2 Treatment of cells by chiral gold nano-dimer under different polarized light

[0028] Polarization-based plasma chiral nano-gold dimer Anti-EGFR-Au dimer-PpIX dimer was centrifuged at 8000rpm for 10min, and the pellet was resuspended in cell culture medium to make the final concentration of Anti-EGFR-Au dimer-PpIX 5nM . Inoculate HeLa cells in a 6-well culture plate so that the number of cells in each well is 10 4 After culturing for 24 hours, remove the culture medium. Add 500μL each of Anti-EGFR-Au dimer-PpIX dimer resuspended in cell culture medium to each well. After culturing for 24 hours, remove the cell culture medium and wash with pH7.4 PBS solution 3 times, and finally add 200 μL of fresh medium. Different culture wells were irradiated with 532nm left-handed, 532nm right-handed and 532nm ordinary light for 30min. It was tested with a cytotoxicity kit.

Embodiment 3

[0029] Example 3 Photodynamic therapy of chiral gold nano-dimer on living tumors under different polarized light

[0030] Centrifuge the obtained Anti-EGFR-Au dimer-PpIX dimer at 8000 rpm for 10 min, and resuspend the pellet in a pH 7.4 PBS solution to make the final concentration of Anti-EGFR-Au dimer-PpIX 50 nM. Three nude mice with xenograft tumors were taken and injected with 100 μL of the Anti-EGFR-Au dimer-PpIX dimer. The tumors of nude mice were irradiated with 532nm left-handed, 532nm right-handed, and 532nm ordinary light for 2h, and the size changes of the tumors were observed and recorded.

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Abstract

The invention discloses a preparation method and application of a plasma chiral nano-gold dimer based on a polarization effect, and belongs to the technical field of material chemistry. The plasma chiral nano-gold dimer based on the polarization effect is prepared by the surface finish of a chiral nano-gold dimer, the coupling of the chiral nano-gold dimer to photosensitive molecules and the coupling of the chiral nano-gold dimer to a targeted antibody; then a killing effect of the chiral nano-gold dimer on cells under different beams of polarized light and a therapeutic effect of the chiral nano-gold dimer on living body tumors under the different beams of polarized light are detected. The invention provides the preparation method of the plasma chiral nano-gold dimer based on the polarization effect, a powerful platform can be provided for selective photodynamic therapy by utilizing the irradiation of different beams of exciting light, and the method can play an important role in the clinical medicine and the biotechnology.

Description

Technical field [0001] The invention relates to a preparation method and application of plasma chiral nano-gold dimer based on polarization effect, and belongs to the technical field of material chemistry. Background technique [0002] Cancer is one of the major diseases threatening human health. The current clinical treatments for cancer are mainly chemotherapy and surgery. These treatments not only have very limited therapeutic effects on target cells, but also cause serious damage to other normal tissues. Emerging treatments based on nanomaterials have great application potential in cancer imaging and phototherapy due to their unique physical and chemical properties. Photodynamic therapy is one of the new technologies that use photodynamic effects to treat patients. The mechanism is that the photosensitizer reacts with the targeted tissues and cells, and then acts after being irradiated with light of a specific wavelength, leading to necrosis of the target cells. [0003] The...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/48A61P35/00
Inventor 徐丽广孙茂忠胥传来匡华马伟刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV
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