Didymella bryoniae fluorescent PCR detection method as well as primer and probe for didymella bryoniae fluorescent PCR detection method
A technology for the pathogenic bacteria and detection methods, which are applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, DNA/RNA fragments, etc. The presence of germs, etc.
Inactive Publication Date: 2015-09-02
新疆出入境检验检疫局检验检疫技术中心
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This method is empirically strong, mainly manifested in: (1) it is difficult to distinguish the shape of P. solan
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The invention discloses primer and probe for a didymella bryoniae fluorescent PCR detection method. The primer includes Db-5: 5'-CGTAAGTAGACCTCCACCAC-3' and Db-7: 5'-CGAAGTGCCATTGTAGACA-3'. The TaqMan probe is Db-P: 5'FAM-TCACTTCTCAACAACAGCGGCTT-3'TAMRA. The invention also provides the didymella bryoniae fluorescent PCR detection method carried out by utilizing the primer and the probe. The didymella bryoniae fluorescent PCR detection method comprises the following steps: extracting the DNA of a to-be-detected plant material; carrying out PCR. According to the didymella bryoniae fluorescent PCR detection method, didymella bryoniae is positive if fluorescent light (Ct value is less than 40) can be detected, otherwise, the didymella bryoniae is negative.
Description
technical field [0001] The invention relates to a fluorescent PCR detection method for the pathogen of melon blight and the used primers and probes. Background technique [0002] Symptoms of melon vine blight: mainly damages a variety of melon vines, but also leaves and fruits. In the initial stage of the vine disease, oil-soaked discolored cracks are produced in the nodes, and glue is secreted. When the condition is severe, the cracks on the vines burst, and gray and red gelatinous liquid will flow out from the cracks at the base of the main vines, commonly known as "exploding blood vessels"; when the side vines are cracked, a white oily substance will be produced. Some plants appear to grow slowly or stop growing; in severe cases, the whole plant dies, and the tendrils turn reddish brown after drying. [0003] The long-distance transmission of melon blight mainly relies on plant materials such as infected plants, diseased residues, and infected seeds. Melon vine blight ...
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Login to view more IPC IPC(8): C12Q1/18C12N15/11
CPCC12Q1/6895C12Q1/6851C12Q2531/113C12Q2563/107
Inventor 王翀孙燕飞雷勇辉朱建军张小菊李亚伟张祥林张伟
Owner 新疆出入境检验检疫局检验检疫技术中心
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