Method for determining hydrogen peroxide based on enzymic catalytic reaction

A technology of hydrogen peroxide and hydrogen peroxide solution, which is applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of reducing resonance scattering intensity and hindering the aggregation of nano-silver, and achieves the effect of good selectivity and high sensitivity

Active Publication Date: 2015-09-02
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bovine hemoglobin (Hb) has the properties of a peroxide mimetic enzyme, which can catalyze H 2 o 2 Oxidation excess I - Generate I 3 - , I 3 - It will hinder the aggregation between nano-silver and reduce the resonance scattering intensity of the system. According to the change of the resonance scattering intensity of the system caused by the change of the nano-silver aggregation state before and after the reaction, a quantitative determination of H can be established. 2 o 2 method, which has not yet been reported

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  • Method for determining hydrogen peroxide based on enzymic catalytic reaction

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Embodiment

[0010] Add 1.0mL pH=4.2 Clark-Lubs (Clark-Lubs, C-L) buffer solution, 1.0mL 0.05mol / L potassium iodide (KI) solution and 0.5mL 2.0×10 -6 mol / L bovine hemoglobin (Hb) solution, then add 0.0, 0.005, 0.01, 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5mL 6.40×10 -5 mol / L hydrogen peroxide solution, after standing at room temperature for 20 minutes, add 0.5mL 2.38×10 -4 mol / L nano-silver, that is, AgNPs solution, was diluted to the mark with twice distilled water, shaken well, and continued to react for 10 minutes, then used a 1cm quartz cuvette to scan the resonance scattering spectrum on a fluorescence spectrophotometer in the form of λex=λem. Measure the resonance scattering intensity I of hydrogen peroxide solution at a wavelength of 430nm 430nm and the resonance scattering intensity I of the reagent blank solution without hydrogen peroxide 0 , to calculate the difference ΔI of the resonant scattering intensities 430nm = I 0 -I 430nm , the difference ΔI of its resonant sc...

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Abstract

The invention discloses a method for determining hydrogen peroxide based on enzymic catalytic reaction. The method comprises the following steps: adding a 0.0-2.0mL Clark-lubs buffer solution with pH equal to 4.2, 0.1-1.5mL of 0.05mol / L potassium iodide and 0.0-1.2mL of 2.0*10<-6>mol / L bovine hemoglobin into 12 10mL colorimetric tubes; then sequentially adding 0.0, 0.005, 0.01, 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5mL of 6.40*10<-5>mol / L H2O2 solutions, and reacting for 20 minutes; then adding 0.1-0.7mL of 2.38*10<-4>mol / L nano silver into each tube, metering the volume to be constant by use of secondary distilled water, further reacting for 10 minutes, and scanning a resonance scattering spectrum by a 1cm quartz colorimetric utensil by a mode of lambda ex=lambda em; determining the scattering intensity I430nm of a solution added with H2O2 at the 430nm part and the scattering intensity I0 of the reagent blank without H2O2, and calculating the difference delta I430nm=I0-1430nm. Pond water or fresh rainwater is additionally taken to be filtered, and the scattering intensity value of the pond water or the fresh rainwater is determined by the same method so as to calculate the content of H2O2 in the pond water or fresh rainwater. The determination method is good in selectivity, and is high in flexibility.

Description

technical field [0001] The invention relates to a method for measuring hydrogen peroxide, in particular to a resonance scattering spectrometry method for measuring hydrogen peroxide based on an enzyme-catalyzed reaction. Background technique [0002] Hydrogen peroxide is a strong oxidant, which can be used as a disinfectant, bleach, bactericide, deoxidizer, etc., and is widely used in food, textile, paper, medicine, chemical, environmental protection, electronics and other fields. Hydrogen peroxide is closely related to human health. Long-term exposure to hydrogen peroxide will cause symptoms such as respiratory tract and skin irritation. The content of hydrogen peroxide in the air should not exceed 1.4mg / m 3 . On the other hand, hydrogen peroxide is one of the main causes of acid rain formation. Therefore, the determination of hydrogen peroxide is of great significance in the fields of environment, chemistry, medicine, and biological sciences. Currently measured H 2 o ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor唐宁莉陈永宁张容珲单雅琦单展覃温露
OwnerGUILIN UNIVERSITY OF TECHNOLOGY