Kit for qualitatively detecting tyrosine metabolites in human urine

A qualitative detection and metabolite technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problems of complicated operation and difficult clinical development, and achieve simple operation methods , Low detection cost, low cost effect

Inactive Publication Date: 2015-09-02
SUZHOU RELING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each method has advantages and disadvantages. For example, the HPLC method for the determination of urinary polyamines has high sensitivity and good selectivity, but the operation is extremely complicated and it is not easy to carry out clinically.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kit for the qualitative detection of tyrosine metabolites in human urine, the kit consists of 1 volume part of 0.5g / mL mercuric chloride solution, 0.5 volume parts of 0.5g / mL mercury nitrate solution and 1 volume part part of 0.1g / mL ferric nitrate solution, the above solution preparation methods are commonly used preparation methods in the prior art, wherein the mercuric chloride solution needs to add a certain amount of sodium chloride in the configuration process, the sodium chloride The amount can be determined according to the mass of mercuric chloride added, generally no more than 1 / 3 of the mass of mercuric chloride.

Embodiment 2

[0021] A kit for the qualitative detection of tyrosine metabolites in human urine, the kit consists of 1 volume part of 0.5g / mL mercuric chloride solution, 1 volume part of 1.0g / mL mercury nitrate solution and 1.5 volume parts part of 0.05g / mL ferric nitrate solution, the above solution preparation methods are commonly used preparation methods in the prior art, wherein the mercuric chloride solution needs to add a certain amount of sodium chloride in the configuration process, the sodium chloride The amount can be determined according to the mass of mercuric chloride added, generally no more than 1 / 3 of the mass of mercuric chloride.

Embodiment 3

[0023] A kit for the qualitative detection of tyrosine metabolites in human urine, the kit consists of 1 volume part of 0.2g / mL mercuric chloride solution, 2 volume parts of 0.5g / mL mercury nitrate solution and 0.5 volume parts part of 0.1g / mL ferric nitrate solution, the above solution preparation methods are commonly used preparation methods in the prior art, wherein the mercuric chloride solution needs to add a certain amount of sodium chloride in the configuration process, the sodium chloride The amount can be determined according to the mass of mercuric chloride added, generally no more than 1 / 3 of the mass of mercuric chloride.

[0024] The using method of product of the present invention is:

[0025] 1. Take fresh clean urine for the first time in the morning;

[0026] 2. The temperature during inspection is about 20°C. Add the above 3ml of urine into the ampule bottle or test tube equipped with the reagent of the present invention, shake well and let stand for 3-5 mi...

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PUM

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Abstract

The invention discloses a kit for qualitatively detecting tyrosine metabolites in human urine. The kit is formed by mixing the following reagents: 0.2-0.5g / mL of mercuric chloride solution, 0.5-1.0g / mL of mercuric nitrate solution, and 0.05-0.1g / mL of ferric nitrate solution, the volume ratio of the mercuric chloride solution to the mercuric nitrate solution to the ferric nitrate solution is 1 to (0.5-2) to (0.5-1.5). The kit disclosed by the invention has the features that the operation method is simple; a special apparatus is not needed; the urine of a patient is adopted for detection, so that the detection is noninvasive, and easy to implement; the result can be instantly obtained; the detection expense is lower than that of the similar method; the kit is suitable for large-scale population screening and therapeutic effect monitoring of cancerous person, and further suitable for the primary hospital.

Description

technical field [0001] The invention relates to a kit for qualitatively detecting tyrosine metabolites in human urine. Background technique [0002] The key to early detection of cancer is regular screening. Among the many tumor markers, protein, hormone or other markers cannot be used as screening markers because they cannot be expressed in a wide enough range of tumors, and most of them are destructive tests. Complex and expensive, it is not suitable for large-scale population screening; detecting tumors through urine avoids the link of blood drawing, which is easy to cause infection, and there are many methods at home and abroad, such as: high performance liquid chromatography (HPLC) Polyamine, ELISA method for determination of urinary ferritin, determination of tyrosine and its metabolites in urine and protein chip detection of tumor markers, etc. Each method has advantages and disadvantages. For example, the HPLC method for the determination of urinary polyamines has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/82
Inventor 扈晓佳梅宗泉
Owner SUZHOU RELING BIOTECH CO LTD
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