Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Nano gene drug capable of stimulating lymphocyte activation and preparation method thereof

A nano and gene technology, applied in gene therapy, drug combination, pharmaceutical formulation, etc., can solve the problems of inability to aggregate, low efficiency of transfecting cells, and unsatisfactory treatment effects of tumors or infectious diseases, etc. Proliferating tumor targeting, enhancing drug efficacy, and prolonging half-life effects

Inactive Publication Date: 2015-09-09
YANGZHOU UNIV
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] When a simple DNA gene drug is applied in vivo, the active uptake efficiency of cells is low, the efficiency of transfection cells is not high, and the expression of related gene products in vivo is affected by the injection route and immune dose
In addition, the expression products of DNA gene drugs generally cannot accumulate in tumors or local infection sites in vivo, resulting in the therapeutic effect on tumors or infectious diseases often not reaching the desired effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nano gene drug capable of stimulating lymphocyte activation and preparation method thereof
  • Nano gene drug capable of stimulating lymphocyte activation and preparation method thereof
  • Nano gene drug capable of stimulating lymphocyte activation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] 1. Construction of pcDNA3.1-sig-dsNKG2D-IL-21 fusion gene eukaryotic expression vector:

[0044] 1.1 Primer sequences for PCR amplification: Using bioinformatics means, design corresponding primers (see Table 1), and amplify signal peptides-sNKG2D(1), sNKG2D(2), IL -21 gene segment. The reverse complementary sequence of (Gly4Ser)4 (shown in the box in the table) was added to the downstream primer of signal peptide-sNKG2D (1) and the downstream primer of sNKG2D (2). figure 2 The construction strategy of the ATG-sig-dsNKG2D-IL-21 fusion gene fragment is shown.

[0045] Table 1. Primer sequences for amplification of signal peptide, NKG2D, and IL-21 gene fragments

[0046]

[0047] The sequence of the PCR amplification product of the signal peptide-sNKG2D(1) is shown in SEQ ID NO: 4, the 1st-6th base is the sequence of the Xba I restriction site; Randomly added nucleotides; No. 8-68 is the gene sequence of mouse β2-microglobulin signal peptide; No. 69-485 is the gene...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a novel nano gene drug capable of stimulating lymphocyte activation and a preparation method thereof. The invention discloses a fusion gene; and a mouse beta-2 microglobulin signal peptide is introduced from the upstream of the fusion gene dsNKG2D-IL-21 gene to obtain the fusion gene ATG-sig-dsNKG2D-IL-21. The invention also discloses a fusion gene expression carrier pcDNA3.1-sig-dsNKg2D-IL-21. The invention also discloses a nano gene vaccine drug which is formed by encapsulating the pcDNA3.1-sig-dsNKg2D-IL-21 fusion protein expression vector with chitosan. The invention also discloses a preparation method of a nano gene vaccine. The nano drug is used for carrying a nano gel of the recombinant dsNKG2D-IL-21 gene vector capable of stimulating lymphocyte activation and in-vivo targeted distribution, and is hopeful to become a drug for resisting tumors and infectious diseases.

Description

technical field [0001] The invention relates to a novel nano-gene drug for stimulating lymphocyte activation and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] The activation of lymphocytes plays an important role in the body's anti-infection and anti-tumor functions. However, in the process of persistent chronic infection or tumorigenesis, the immune function of the body is suppressed and is in a low activity state. The preparation of effective drugs for stimulating lymphocyte activation is of great significance for the effective treatment of chronic infectious diseases and tumors. The growth and activation of lymphocytes mainly depend on cytokines, such as IL-2, IL-7, IL-15, IL-21, etc. Therefore, based on the genes and protein sequences of cytokines, the corresponding genes and proteins are prepared in vitro, theoretically It has a good function of promoting lymphocyte proliferation and stimulating lymphocyte activat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/62C12N15/63A61K48/00A61K39/00A61P35/00A61P37/02
Inventor 赵培蕾龚卫娟肖炜明
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com