Application of sodium valproate in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
A technology of plant pathogenic bacteria and sodium valproate, which is applied in the field of fungicides for plant diseases, can solve the problems of reports on the inhibitory effect of sodium valproate, and achieve good inhibitory activity, large pollution, and high residue effects
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[0037] 1. Experimental materials
[0038] Sodium valproate was purchased as a finished product from Sigma Corporation. Weigh 3.32g of sodium valproate in 20ml of distilled water to prepare a 1M mother liquor, filter and sterilize and store at 4°C until use.
[0039] The phytopathogenic bacteria used in this experiment were strains stored at 4°C in the laboratory, and the medium used was potato medium (PDA for short).
[0040] PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural pH.
[0041] Preparation method: wash and peel the potatoes, weigh 200g, cut into small pieces, add water and boil until rotten (boil for 20-30 minutes, it can be pierced by a glass rod), filter it into a beaker with eight layers of gauze, add 15-20g of agar, add 20g of glucose, stir evenly, fully dissolve and then cool down to make up water to 1000ml, after subpackaging, sterilize at 121°C for 20 minutes, cool down and set aside.
[0042] 2. Experimental method ...
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