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Method for detecting sheep FecB gene polymorphism by use of SNaPshot technology

A technical detection and sheep technology, which is applied in the field of molecular marker detection, can solve the problems of complicated procedures, difficult high-throughput automatic determination, and low throughput, and achieve the effects of high technical accuracy, improved fecundity, and low cost

Inactive Publication Date: 2015-09-23
内蒙古河套农牧业技术研究院
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AI Technical Summary

Problems solved by technology

Most of the traditional FecB gene detection methods use PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) detection methods. Difficult to achieve high-throughput automated assays

Method used

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  • Method for detecting sheep FecB gene polymorphism by use of SNaPshot technology
  • Method for detecting sheep FecB gene polymorphism by use of SNaPshot technology
  • Method for detecting sheep FecB gene polymorphism by use of SNaPshot technology

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 Utilizes the method of SNaPshot technology to detect sheep FecB gene polymorphism

[0037] 1. Experimental materials

[0038] 146 Luxi black-headed sheep were selected as test objects.

[0039] 2. Reagents and instruments

[0040] Dongshenglong Black King Kong EDC-810 PCR instrument was purchased from Beijing Dongsheng Innovation Biotechnology Co., Ltd., 3730XL gene sequencer was purchased from ABI Branch of the United States, Allegra 25R desktop high-speed refrigerated centrifuge was purchased from American Beckman Company, Micro 17R micro desktop centrifuge The micro-adjustable pipette was purchased from Eppendorf Company in Germany, Taq DNA polymerase, dNTP, ExoI enzyme and FastAP enzyme were purchased from Fermentas Company in Canada, and the SNaPshot kit was purchased from ABI Company in the United States.

[0041] 3. Genomic DNA extraction

[0042]1ml of blood was collected from the jugular vein of the sheep and treated with EDTA anticoagulant. Fi...

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Abstract

The invention provides a method for detecting sheep FecB gene polymorphism by use of the SNaPshot technology. The method is to conduct single nucleotide polymorphism detection on the 29382188bp site (NC_019463.1 on the sixth chromosome of sheep, based on sheep genomic sequence information version number Oar_v3.1, Dec, 2012) and judging whether the sheep FecB gene is homozygous or heterozygous according to the detection result. Compared with the traditional PCR-RFLP FecB gene detection method and other methods, the method provided by the invention is high in technical accuracy, quick in detection, low in cost, and easier in result identification, and can be used for automatically detecting the SNP site of the FecB gene. Through selection of the sheep FecB genetypes, GG homozygote individuals with high litter size character can be reserved to improve the sheep reproductive capacity, and the method has a potential application value in large-scale sheep breeding.

Description

technical field [0001] The invention relates to molecular marker detection technology, in particular to a method for detecting sheep FecB gene polymorphism by using SNaPshot technology. Background technique [0002] Livestock litter size is a quantitative trait with enormous economic value. The selection of lamb size is not only restricted by sex and age, but also the lamb size of sheep is a quantitative trait with low heritability, so it is difficult to improve the litter size trait with conventional breeding techniques. Marker-assisted selection can greatly improve the selection efficiency of such low-heritability traits by affecting the selection time, intensity and accuracy of selection, and finding molecular genetic markers linked to these quantitative trait loci is the key to realizing marker-assisted selection. Selected prerequisites. [0003] In the 1950s, the Seears brothers bred a group of Australian Merino (Booroola Merino, BM) that could give birth to multiple ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 储明星刘秋月狄冉胡文萍王翔宇王贵潘章源赵万民李虎山
Owner 内蒙古河套农牧业技术研究院
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