Method for pollen fermentation with bacillus coagulans and its fermentation product and application
A technology of Bacillus coagulans and fermentation products, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve the problems of high equipment requirements and high condition control requirements, avoid freeze-drying, easy to colonize the intestinal tract, The effect of strong stomach acid resistance
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Embodiment 1
[0042] 1. Inoculate the preserved strains on the slant strain culture medium, and carry out activation culture at 30°C for 48 hours to obtain the slant strains. The slant culture medium (1L) is: peptone 5g, yeast extract 5g, diammonium hydrogen citrate 1g, glucose 10g, Tween-800.5mL, sodium acetate 1g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.1g, Manganese sulfate 0.1g, agar 15g, distilled water 1000mL, pH 6.
[0043] 2. Wash the slanted bacteria with sterile water to make a bacterial suspension, and adjust its OD600 to 1.0.
[0044]3. The bacterial suspension was transferred to the liquid strain medium at a ratio of 5% (v / v), and cultured at 30° C. and 100 r / min for 12 hours to obtain liquid strains. The liquid culture medium (1L) is: peptone 5g, yeast extract 1g, diammonium hydrogen citrate 1g, glucose 10g, Tween-80 0.5mL, sodium acetate 1g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.1g , manganese sulfate 0.1g, distilled water 1000mL, pH 6.
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Embodiment 2
[0048] 1. Inoculate the preserved strains on the slant strain culture medium, and carry out activation culture at 37°C for 24 hours to obtain the slant strains. The slant culture medium (1L) is: peptone 10g, yeast extract 10g, diammonium hydrogen citrate 2g, glucose 15g, Tween-801mL, sodium acetate 4g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.6g, sulfuric acid Manganese 0.5g, agar 17g, distilled water 1000mL, pH 6.
[0049] 2. Wash the slanted bacteria with sterile water to make a bacterial suspension, and adjust its OD600 to 1.0.
[0050] 3. The bacterial suspension was transferred to the liquid strain culture medium at a ratio of 7% (v / v), and cultured at 35° C. and 150 r / min for 24 hours to obtain liquid strains. The liquid culture medium (1L) is: peptone 10g, yeast extract 10g, diammonium hydrogen citrate 2g, glucose 15g, Tween-80 1mL, sodium acetate 4g, dipotassium hydrogen phosphate 2g, magnesium sulfate 0.6g, Manganese sulfate 0.5g, distilled water 1000m...
Embodiment 3
[0054] 1. Inoculate the preserved strains on the slant strain culture medium, and carry out activation culture at 40°C for 12 hours to obtain the slant strains. The slant culture medium (1L) is: peptone 15g, yeast extract 15g, diammonium hydrogen citrate 3g, glucose 30g, Tween-802mL, sodium acetate 9g, dipotassium hydrogen phosphate 3g, magnesium sulfate 1g, manganese sulfate 1g, 20g agar, 1000mL distilled water, pH 7.
[0055] 2. Wash the slanted bacteria with sterile water to make a bacterial suspension, and adjust its OD600 to 1.0.
[0056] 3. The bacterial suspension was transferred to the liquid strain culture medium at a ratio of 10% (v / v), and cultured at 40° C. and 200 r / min for 30 hours to obtain liquid strains. The liquid culture medium (1L) is: peptone 15g, yeast extract 10g, diammonium hydrogen citrate 3g, glucose 30g, Tween-80 2mL, sodium acetate 9g, dipotassium hydrogen phosphate 3g, magnesium sulfate 1g, sulfuric acid Manganese 1g, distilled water 1000mL, pH 7...
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