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Method for synthesizing biological agent capable of enriching heavy metal cadmium in rice field

A technology of biological preparations and synthetic methods, which is applied in the fields of site preparation, restoration of polluted soil, agricultural machinery and implements, etc., can solve the problems of large area of ​​cadmium pollution, low content in soil, and incomplete removal, etc., to achieve environmental friendliness, Effect of improving removal rate and reducing cost

Inactive Publication Date: 2015-10-07
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention: in view of the large area of ​​cadmium pollution in the current paddy field and the low content in the soil, the currently commonly used phytoremediation technology only reduces the mobility of cadmium in the soil, and does not completely remove the cadmium in the soil. content, and proposed a method for synthesizing plants to absorb biological agents. The biological agent is injected into the roots of water lettuce to promote the enrichment of micro-polluted heavy metal cadmium in the roots of water lettuce. With the rapid reproduction of water lettuce, it is effective The pollution of cadmium in the soil of paddy fields has been effectively controlled, and the harm to the human body caused by the heavy metal cadmium in the soil enriched by rice has been avoided.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] First, take 6g of gallinaceous cane stems, add 20mL of ethanol to heat and reflux, reflux twice, each time for 2 hours, combine the extracts, concentrate and evaporate to dryness; then add 25mL of ethyl acetate, extract the liquid after ultrasonic oscillation for 25min, and extract the extract After evaporating to dryness, add 8 mL of methanol to dissolve; then add 6 mL of cockscomb blood to the above mixed solution and mix evenly, and separate under the action of centrifugal force to obtain the clear liquid; then inject the separated clear liquid into the root of water lettuce with a pinhole , the injection volume of each piece of water floating lotus is 2mL; collect 60 pieces of water floating lotus that have been injected with the drug, remove the root and dry it, and then crush it into powder; add 15mL ethyl acetate to the above powder, and extract it for 4 hours to obtain Extract: put the above extract in a 60mL stoppered colorimetric tube, place it in water at a co...

example 2

[0027] First, take 10g of Spathiphyllum vine and add 23mL of ethanol to heat and reflux, reflux twice for 2.5 hours each time, combine the extracts, concentrate and evaporate to dryness; then add 30mL of ethyl acetate, extract the liquid after ultrasonic oscillation for 28min, and extract the extract After evaporating to dryness, add 10 mL of methanol to dissolve; then add 8 mL of cockscomb blood to the above mixed solution and mix evenly, and separate under the action of centrifugal force to obtain the clear liquid; then inject the separated clear liquid into the root of water lettuce with a pinhole , the injection volume of each piece of water floating lotus is 2.5mL; collect 80 pieces of water floating lotus that have been injected with the drug, remove the root and dry it, and then crush it into powder; add 18mL ethyl acetate to the above powder, and extract it for 4.5h Obtain the extract; put the above extract into a 60mL stoppered colorimetric tube, place it in water at 7...

example 3

[0031] Firstly, take 15g of gallinaceous cane and add 25mL of ethanol to heat and reflux, reflux twice, each time for 3 hours, combine the extracts, concentrate and evaporate to dryness; then add 45mL of ethyl acetate, extract the liquid after ultrasonic vibration for 30min, and extract the extract After evaporating to dryness, add 12 mL of methanol to dissolve; then add 10 mL of cockscomb blood to the above mixed solution and mix evenly, and separate under the action of centrifugal force to obtain the clear liquid; then inject the separated clear liquid into the root of water lettuce with a pinhole , the injection volume of each water floating lotus is 3mL; then collect 90 pieces of water floating lotus that have been injected with the drug, remove the root and dry it, and then crush it into powder; add 20mL ethyl acetate to the above powder, and extract it for 5 hours to obtain Extract: put the above extract in a 60mL stoppered colorimetric tube, place it in water at 80°C for...

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Abstract

The invention relates to a method for synthesizing a biological agent capable of enriching heavy metal cadmium in a rice field and belongs to the technical field of ecological remediation of heavy metal polluted soil. The biological agent provided by the invention is injected into roots of water lettuces to promote the enriching effect of the roots of the water lettuces to micro pollutant heavy metal cadmium, the pollution caused by cadmium in the rice field is effectively controlled with the quick reproduction of the water lettuces and the harm caused to human bodies by heavy metal cadmium enriched by rice in the soil is avoided. According to feedback of research and example results, the biological agent can finally enable the enrichment factor of cadmium in the water lettuces to reach more than 10000 and the removal rate of cadmium to highly reach more than 99.6 percent at the same time.

Description

technical field [0001] The invention relates to a biological preparation method for enriching heavy metal cadmium content in paddy fields, and belongs to the technical field of heavy metal soil ecological restoration. Background technique [0002] As a toxic heavy metal, cadmium has become more and more polluting to crops, and the polluted area of ​​farmland is expanding day by day, which has attracted the attention of the society. In addition to causing acute and chronic poisoning of humans and animals, cadmium also has a strong carcinogenic effect. In recent years, due to the large-scale use of chemical fertilizers and pesticides in industry, heavy metal pollution in farmland has intensified, and the safety of heavy metals in rice has become increasingly prominent. According to the Ministry of Agriculture, rice and The product quality supervision and inspection department conducted a general survey on rice safety, showing that one of the quality and safety problems of rice ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/00A01B79/02
Inventor 雷春生张帆
Owner CHANGZHOU UNIV
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