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Method for improving yield of 10-deacetylbaccatin III produced by fungi

A technology of acetylbaccatin and yield, which is applied in the field of improving the yield of 10-desacetylbaccatin III produced by fungi, can solve the problem of low yield of 10-desacetylbaccatin III, and reduce extraction and purification costs, reduce Effects of pollution and yield improvement

Inactive Publication Date: 2015-10-14
HENAN INST OF SCI & TECH
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for improving the yield of 10-deacetylbaccatin III produced by fungi, which solves the problem of low yield of 10-deacetylbaccatin III after the fermentation of the original endophyte existing in the prior art

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  • Method for improving yield of 10-deacetylbaccatin III produced by fungi

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Embodiment Construction

[0021] The present invention will be described in detail below in combination with specific embodiments.

[0022] The invention provides a method for improving the yield of 10-desacetylbaccatin III produced by fungi, comprising the following steps:

[0023] Trichoderma sp. strain IRB54 was cultivated in the culture medium. After the mycelium was fermented and cultivated for 3 days, the cycle culture was carried out at 10°C for 6 hours, 25°C for 12 hours, and 30°C for 6 hours, so that the growth of the strain IRB54 was stimulated by high and low temperature. Thereby increasing the production of fungal secondary matter.

[0024] Among them, the medium is PDB medium, and its composition is: water 1000ml, potato 600g, sucrose 280g, pH6.5-7.0;

[0025] The cultivation process is as follows: firstly, culturing at 25° C. for 3 days, and the rotation speed of the cultivation shaker is 230r / min-250r / min. The initial temperature of the temperature cycling culture is low temperature 10...

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Abstract

The invention discloses a method for improving yield of 10-deacetylbaccatin III produced by fungi. The method comprises the following steps: culturing in a culture medium to produce 10-deacetylbaccatin III, specifically, culturing at 25 DEG C for three days, then culturing at 10 DEG C for six hours, then culturing at 25 DEG C for 12 hours, and then culturing at 30 DEG C for six hours, and circulating like this at 12 variable temperatures. Therefore, the yield of 10-deacetylbaccatin III produced by fungi is stimulated by circulating excitation of high and low temperatures. Compared with the traditional culturing method, the method provided by the invention has the advantages that the yield of 10-deacetylbaccatin III is obviously improved, the operation is simple, the production cost is low, the purpose of improving the yield is achieved simply by changing the temperature, and the yield of 10-deacetylbaccatin III can be improved by 2.4 times.

Description

technical field [0001] The invention belongs to the technical field of fungi cultivation, and in particular relates to a method for increasing the yield of 10-desacetylbaccatin III produced by fungi. Background technique [0002] Paclitaxel is currently the best anti-cancer chemotherapy drug, especially for women with high incidence of breast cancer, uterine cancer and ovarian cancer. However, due to its complicated artificial synthesis process and high price, the drug is mainly extracted from Taxus chinensis. The paclitaxel content of Taxus chinensis is extremely low, and the growth of this plant is extremely slow. Therefore, obtaining paclitaxel artificially and semi-synthetically through baccatin III or 10-desacetyl baccatin III is currently the main way of this drug. Although the content of baccatin III or 10-desacetylbaccatin III in Taxus chinensis is higher than that of paclitaxel, it still cannot meet the market demand, so the price of paclitaxel is still expensive an...

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Application Information

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IPC IPC(8): C12P17/18C07D305/14C12R1/885
Inventor 周修任李勇超代磊杨靖简在友王鸿升许桂芳孟丽
Owner HENAN INST OF SCI & TECH
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