Method for improving yield of 10-deacetylbaccatin III produced by fungi
A technology of acetylbaccatin and yield, which is applied in the field of improving the yield of 10-desacetylbaccatin III produced by fungi, can solve the problem of low yield of 10-desacetylbaccatin III, and reduce extraction and purification costs, reduce Effects of pollution and yield improvement
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[0021] The present invention will be described in detail below in combination with specific embodiments.
[0022] The invention provides a method for improving the yield of 10-desacetylbaccatin III produced by fungi, comprising the following steps:
[0023] Trichoderma sp. strain IRB54 was cultivated in the culture medium. After the mycelium was fermented and cultivated for 3 days, the cycle culture was carried out at 10°C for 6 hours, 25°C for 12 hours, and 30°C for 6 hours, so that the growth of the strain IRB54 was stimulated by high and low temperature. Thereby increasing the production of fungal secondary matter.
[0024] Among them, the medium is PDB medium, and its composition is: water 1000ml, potato 600g, sucrose 280g, pH6.5-7.0;
[0025] The cultivation process is as follows: firstly, culturing at 25° C. for 3 days, and the rotation speed of the cultivation shaker is 230r / min-250r / min. The initial temperature of the temperature cycling culture is low temperature 10...
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