A Micro-droplet Fusion Method Based on Hydrophilic Filament Induction

Abstract

The invention relates to a micro-fluidic technology, and particularly relates to a micro-droplet fusion method based on hydrophilic cellosilk induction. Specifically, hydrophilic cellosilk is embedded into a micro-channel of a chip, a surfactant is mixed into an oil phase, when micro-droplets on two wings of the chip flow through the hydrophilic cellosilk, the parts, contact with the hydrophilic cellosilk, of the micro-droplets can be extended and deformed, so that water-oil interfaces of the micro-droplets are unstable, and then the micro-droplet fusion is triggered. The micro-droplet fusion method provided by the invention is easy to operate, and can efficiently achieve stable droplet fusion of the surfactant without requiring the aid of any external energy sources.

Description

technical field

[0001] The invention relates to microfluidic technology, in particular to a micro-droplet fusion method induced by hydrophilic fiber filaments. Background technique

[0002] Micro-droplets are formed under the interaction of flow shear force and surface tension by taking advantage of the immiscibility of water and oil. Highly monodisperse microdroplets can be produced on a chip in a high-throughput and controllable manner using microfluidic technology. As an independent reactor, the microdroplet has the characteristics of small volume (picoliter to nanoliter), low risk of diffusion and cross-contamination between samples, and rapid mixing of inclusions. At present, micro-droplet technology has been widely used in the synthesis of new materials, cell culture and screening, protein crystallization, detection of biomarkers and other fields.

[0003] For chemical and biological reaction systems, the amount and order of addition of reagents are directly related ...

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