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Synthetic method for biological preparation capable of enriching content of heavy metal mercury in paddy field

A technology of biological preparations and synthesis methods, which is applied in water pollutants, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of reducing mercury mobility, large area of ​​mercury pollution, and incomplete removal, and achieves environmental friendliness. , The effect of improving removal rate and reducing cost

Inactive Publication Date: 2015-11-04
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention: In view of the large area of ​​mercury pollution in the rice field and the low content in the soil, the currently commonly used phytoremediation technology only reduces the mobility of mercury in the soil, and does not completely remove the mercury in the soil. content, and proposed a method for synthesizing plants to absorb biological agents. The biological agent is injected into the roots of water lettuce to promote the enrichment of micropolluted heavy metal mercury in the roots of water lettuce. With the rapid reproduction of water lettuce, it is effective The pollution of mercury in the soil of paddy fields has been effectively controlled, and the harm to the human body caused by the heavy metal mercury in the soil enriched by rice has been avoided.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] First, take 5g of golden steel rattan and crush it, add 15mL of ethanol to heat and reflux, reflux twice, each time for 2 hours, combine the extracts, concentrate and evaporate to dryness; then add 30mL of ethyl acetate, extract the liquid after ultrasonic oscillation for 20min, and extract the extract After evaporating to dryness, add 5 mL of methanol to dissolve; then add 5 mL of cockscomb blood to the above mixed solution and mix evenly, and separate under the action of centrifugal force to obtain the clear liquid; then inject the separated clear liquid into the root of water lettuce through a pinhole, The injection volume of each water floating lotus is 2mL; next, collect 50 pieces of water floating lotus that have been injected with the drug, remove the roots and dry them, and then crush them into powder; Extract solution; then put the above extract solution in a 50mL stoppered colorimetric tube, place it in water at 68°C for 1 hour, add 3g agar and 1g protein, mix ...

example 2

[0028] First, take 8g of golden steel rattan and crush it, add 20mL of ethanol to heat and reflux, reflux twice, each time for 2.5 hours, combine the extracts, concentrate and evaporate to dryness; then add 40mL of ethyl acetate, extract the liquid after ultrasonic oscillation for 25min, and extract the extract After evaporating to dryness, add 8 mL of methanol to dissolve; then add 7 mL of cockscomb blood to the above mixed solution and mix evenly, and separate under the action of centrifugal force to obtain the clear liquid; then inject the separated clear liquid into the root of water lettuce through a pinhole, The injection volume of each piece of water floating lotus is 2.5mL; next, collect 70 pieces of water floating lotus that have been injected with the drug, remove the roots and dry them, and then crush them into powder; add 15mL ethyl acetate to the above powder, and extract for 5.5h The extract was obtained; then the above extract was placed in a 50mL stoppered color...

example 3

[0032]First, take 10g of golden steel rattan and crush it, add 25mL of ethanol to heat and reflux, reflux twice, each time for 3 hours, combine the extracts, concentrate and evaporate to dryness; then add 50mL of ethyl acetate, extract the liquid after ultrasonic oscillation for 30min, and extract the extract After evaporating to dryness, add 10 mL of methanol to dissolve; then add 10 mL of cockscomb blood to the above mixed solution and mix evenly, and separate under the action of centrifugal force to obtain the clear liquid; then inject the separated clear liquid into the root of water lettuce through a pinhole, The injection volume of each piece of water floating lotus is 3mL; next, collect 100 pieces of water floating lotus that have been injected with the drug, remove the roots, dry them, and then crush them into powder; add 20mL of ethyl acetate to the above powder, extract for 6 hours to obtain Extraction solution; then put the above extraction solution in a 50mL stopper...

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PUM

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Abstract

The invention relates to a synthetic method for a biological preparation capable of enriching the content of heavy metal mercury in a paddy field, belonging to the technical field of ecological restoration of heavy metal contaminated soil. The biological preparation provided by the invention is injected into water lettuce roots, so enrichment of micropollution heavy metal mercury in the water lettuce roots is promoted; with rapid proliferation of the water lettuce, mercury pollution in the soil of the paddy field is effectively controlled; thus, hazards to human body due to enrichment of the heavy metal mercury in the soil of the paddy field are avoided. According to feedback of research and example results, with the biological preparation in the invention, enrichment factors of the mercury in the water lettuce can ultimately reach more than 12000; meanwhile, removal rate of the mercury reaches up to more than 99.7%.

Description

technical field [0001] The invention relates to a method for synthesizing biological preparations for enriching heavy metal mercury content in paddy fields, and belongs to the technical field of heavy metal soil ecological restoration. Background technique [0002] Mercury, as a toxic heavy metal, has become more and more polluting to crops, and the polluted area of ​​farmland is expanding day by day, which has attracted attention from the society. In addition to causing acute and chronic poisoning of humans and animals, mercury also has a strong carcinogenic effect. In recent years, due to the large-scale use of chemical fertilizers and pesticides in the industry, heavy metal pollution in farmland has intensified, and the safety of heavy metals in rice has become increasingly prominent. According to the Ministry of Agriculture, rice and The product quality supervision and inspection department conducted a general survey on rice safety, showing that one of the quality and saf...

Claims

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Application Information

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IPC IPC(8): C02F3/32C02F101/20
Inventor 雷春生张帆
Owner CHANGZHOU UNIV
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