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Application of Nobiletin

A technology of tangerine peel and health products, applied in the fields of compound use and citrus flavonoids use, can solve problems such as the prevention and treatment of unseen oral tumors, and achieve the effects of convenient consumption and clinical application, small effective dose, and easy acquisition.

Active Publication Date: 2018-04-13
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, nobiletin has been confirmed to have anti-tumor, anti-virus, hypoglycemic, neuroprotective and other biological activities. Its anti-cancer activity is mainly reported for breast cancer, liver cancer, gastric cancer, lung cancer, etc., but it has not been used for oral tumors. prevention and treatment of

Method used

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  • Application of Nobiletin
  • Application of Nobiletin
  • Application of Nobiletin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Effect of nobiletin on the morphology of human oral epidermoid carcinoma KB cells

[0028] KB cells treated with nobiletin for 48 hours were observed under an inverted microscope, as shown in figure 1 shown. Normal untreated KB cells were polygonal, with clear cytoplasm and vigorous cell growth. After being treated with nobiletin for 48 hours, the number of KB cells decreased significantly, the cells shrunk, the volume decreased, the cell shape could not be distinguished, and the number of apoptotic cells increased with the dose increasing.

Embodiment 2

[0029] Example 2: Inhibitory effect of nobiletin, nobiletin and naringenin on human oral epidermoid cancer cell KB.

[0030] The inhibitory effect of nobiletin, nobiletin and naringenin on human oral epidermoid cancer cells was investigated by the method of methylene blue counting. The inhibitory effect curve of each compound on the proliferation of KB cells is as follows: figure 2 shown.

[0031] The experimental results showed that nobiletin, nobiletin and naringenin had a strong inhibitory effect on the proliferation of human oral epidermoid cancer cells, and each dose group showed a better dose-effect relationship. EC of each compound 50 The values ​​are shown in Table 1, in which nobiletin showed the best activity against oral epidermal carcinoma cells, and its EC 50 The value is 24.60±1.07μM, which is lower than the concentrations of other drug groups.

[0032] Table 1. EC of nobiletin, nobiletin and naringenin on human oral epidermal cancer cells 50 value result

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Embodiment 3

[0034] Example 3: Research on Nobiletin's Transcriptional Regulation of Oral Epidermal Cells

[0035] Real-time quantitative PCR was used to detect the regulation of nobiletin on the transcription level of oral epidermoid cancer cells, and the genes with significant changes were as follows: figure 2 shown.

[0036] The experimental results show that nobiletin can significantly up-regulate the gene expression of tumor suppressor protein p53 and proteolytic enzyme caspase-3, thereby initiating programmed cell death and inhibiting the proliferation of cancer cells. Nobiletin can significantly inhibit the gene expression of inflammatory factor IL-8 and matrix metalloproteinase MMP2, indicating that it can further regulate the immune regulation, invasion and metastasis of cancer cells. It can also be seen from the results that nobiletin has a significant regulatory effect on the transcription level of oral cancer cells, thereby inhibiting the proliferation of oral cancer cells an...

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Abstract

The invention discloses the application of nobiletin, in particular the application in the preparation of health products or medicines for preventing and / or treating oral cancer. The antiproliferative effects of nobiletin, naringenin and nobiletin on the human oral epidermoid cancer cell through the monomeric compounds nobiletin, naringenin and nobiletin in the citrus fruits of Rutaceae showed that nobietin, naringenin and nobiletin had the effect on human oral epidermoid cancer cells. The proliferation of cancer cells is obviously inhibited, and nobiletin has the most obvious effect of inhibiting the proliferation of oral cancer cells. It can be used to prepare health care products and medicines that can prevent and treat oral cancer, and is suitable for large-scale promotion.

Description

technical field [0001] The present invention relates to the technical field of application of compounds, more specifically, to the technical field of application of citrus flavonoids, in particular to the application of nobiletin in health care products and medicines for the prevention and treatment of oral cancer. Background technique [0002] Oral cancer is one of the common malignant tumors in the head and neck, accounting for about 3% of systemic malignant cancers and seriously endangering human health. Oral squamous carcinoma, also known as oral epidermal carcinoma, accounts for 90% of oral cancers. At present, the treatment of oral cancer is mainly based on surgery, supplemented by radiotherapy and chemotherapy, but these will cause oral and maxillofacial tissue and organ defects, resulting in dysfunction of speech, chewing, swallowing, etc., seriously affecting the quality of life of patients. In addition, the prognosis of oral squamous carcinoma patients after treat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/49A61K31/352A61K9/20A61Q11/00A61P35/00A61P11/04A23G4/12
Inventor 陈谷王宏
Owner SOUTH CHINA UNIV OF TECH
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