Application of Nobiletin
A technology of tangerine peel and health products, applied in the fields of compound use and citrus flavonoids use, can solve problems such as the prevention and treatment of unseen oral tumors, and achieve the effects of convenient consumption and clinical application, small effective dose, and easy acquisition.
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Embodiment 1
[0027] Example 1: Effect of nobiletin on the morphology of human oral epidermoid carcinoma KB cells
[0028] KB cells treated with nobiletin for 48 hours were observed under an inverted microscope, as shown in figure 1 shown. Normal untreated KB cells were polygonal, with clear cytoplasm and vigorous cell growth. After being treated with nobiletin for 48 hours, the number of KB cells decreased significantly, the cells shrunk, the volume decreased, the cell shape could not be distinguished, and the number of apoptotic cells increased with the dose increasing.
Embodiment 2
[0029] Example 2: Inhibitory effect of nobiletin, nobiletin and naringenin on human oral epidermoid cancer cell KB.
[0030] The inhibitory effect of nobiletin, nobiletin and naringenin on human oral epidermoid cancer cells was investigated by the method of methylene blue counting. The inhibitory effect curve of each compound on the proliferation of KB cells is as follows: figure 2 shown.
[0031] The experimental results showed that nobiletin, nobiletin and naringenin had a strong inhibitory effect on the proliferation of human oral epidermoid cancer cells, and each dose group showed a better dose-effect relationship. EC of each compound 50 The values are shown in Table 1, in which nobiletin showed the best activity against oral epidermal carcinoma cells, and its EC 50 The value is 24.60±1.07μM, which is lower than the concentrations of other drug groups.
[0032] Table 1. EC of nobiletin, nobiletin and naringenin on human oral epidermal cancer cells 50 value result
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Embodiment 3
[0034] Example 3: Research on Nobiletin's Transcriptional Regulation of Oral Epidermal Cells
[0035] Real-time quantitative PCR was used to detect the regulation of nobiletin on the transcription level of oral epidermoid cancer cells, and the genes with significant changes were as follows: figure 2 shown.
[0036] The experimental results show that nobiletin can significantly up-regulate the gene expression of tumor suppressor protein p53 and proteolytic enzyme caspase-3, thereby initiating programmed cell death and inhibiting the proliferation of cancer cells. Nobiletin can significantly inhibit the gene expression of inflammatory factor IL-8 and matrix metalloproteinase MMP2, indicating that it can further regulate the immune regulation, invasion and metastasis of cancer cells. It can also be seen from the results that nobiletin has a significant regulatory effect on the transcription level of oral cancer cells, thereby inhibiting the proliferation of oral cancer cells an...
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