The New Application of Elbowtocin Compounds
A technology of Elbow topertocin and compound, which is applied in the field of new medical use of Elbow topertoin compounds, and can solve the problems of preventing and/or treating obesity, diabetes and the like that have not been found.
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Embodiment 1
[0040] Embodiment 1, the preparation of myelin compounds
[0041] The elbow mycin compound involved in the present invention is prepared by fermentation of Aspergillus deflectus NCC0415. The Aspergillus deflectus strain NCC0415 has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation date is 2014. On April 25, the deposit number was CGMCCNo.9095, and the deposit address was: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
[0042] (a) prepare the seed solution of Aspergillus deflectus (Aspergillus deflectus) NCC0415
[0043] Aspergillus deflectus (Aspergillus deflectus) fungus NCC0415 was inoculated in the seed medium, and cultured at 25°C at 200 rpm for 72 hours to obtain seed liquid. The preparation method of the seed medium is: 20.0 grams of corn starch, 10.0 grams of glucose, 4.0 grams of hot-pressed soybean cake powder...
Embodiment 2
[0066] Example 2 Determination of Inhibitory Activity of MEG2, SHP1, SHP2 and PTP1B
[0067] MEG2, SHP1, SHP2 and PTP1B are all recombinant human protein tyrosine phosphatases expressed in Escherichia coli by genetic engineering methods and purified by affinity chromatography.
[0068] The method for measuring the inhibitory activity is as follows: Dilute the elbow mycin compounds prepared in Example 3 and add them to a 96-well plate, and add 100 μl of reaction buffer containing any enzyme protein of MEG2, SHP1, SHP2 or PTP1B to each well After incubation at room temperature for 15 minutes, 100 μl of buffer containing 5 mM disodium p-nitrophenylphosphate (pNPP) reaction substrate was added. The reaction was incubated at 37°C for 30min, and 0.2M NaOH was added to terminate the reaction, and the light absorption change at 405nm was measured with a microplate reader.
[0069] The inhibition rate was calculated according to the following formula:
[0070] Inhibition rate=(1-aver...
Embodiment 3
[0078] Embodiment 3 has the preparation of the pharmaceutical composition of PTP inhibitory effect
[0079] Weigh 10 g of the elbow mycetin compound prepared in Example 1, add 50 g of sucrose and 100 g of dextrin, mix well, add ethanol to make wet granules, dry, add 2 g of micropowder silica gel, mix well again, pack into the capsule shell, Make 500 pills of medicine with PTP inhibitory effect.
[0080] Using the compound of the present invention as the active ingredient, it can be prepared according to methods known in the art, and can be prepared into various clinically required preparations, which will not be repeated here.
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