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Colloidal gold immunochromatographic assay test strip for detecting dexamethasone residues in milk

A technology of immunochromatographic test paper and dexamethasone, which is applied in anti-animal/human immunoglobulin, biological testing, anti-hormone immunoglobulin, etc., can solve problems such as glucocorticoid residues and human health hazards, and achieve easy Operation, easy operation, high sensitivity effect

Inactive Publication Date: 2015-11-11
WUHAN SHANGCHENG BIOTECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Because the unreasonable use of such drugs may lead to the residue of glucocorticoids in animal-derived foods, it constitutes a potential hazard to human health

Method used

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  • Colloidal gold immunochromatographic assay test strip for detecting dexamethasone residues in milk
  • Colloidal gold immunochromatographic assay test strip for detecting dexamethasone residues in milk
  • Colloidal gold immunochromatographic assay test strip for detecting dexamethasone residues in milk

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Embodiment 1

[0029] The preparation of embodiment 1 colloidal gold immunochromatography test strip

[0030] 1.1 Preparation and purification of monoclonal antibodies

[0031] 1.1.1 Cell fusion and screening: Immunization qualified mice were given booster immunization 3 days before fusion. According to the counting results of myeloma cells, mix myeloma cells with immune spleen cells; centrifuge at 1500r / min for 5min, pour out the supernatant of the centrifuge tube, turn it upside down on absorbent paper, and control the water droplets; Insert a 1mL graduated pipette of 50% polyethylene glycol (PEG) warmed to 37°C into the bottom of the tube, slowly add PEG to the mixed cells, stir gently while adding, let stand for 90 sec after the addition, and use a pre-warmed to RPMI-1640 basal culture medium (purchased from Hyclone Company) at 37°C was slowly added to the fused cells along the tube wall, and the centrifuge tube was gently shaken while adding. Centrifuge at 1500r / min for 5min, discard ...

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Abstract

The invention belongs to the technical field of veterinary drug residue analysis and particularly relates to a colloidal gold immunochromatographic assay test strip for detecting dexamethasone residues in milk. The test strip is formed by sequentially stacking and adhering an absorbing cushion, a nitrocellulose membrane, a gold standard cushion and a sample cushion to a plastic liner plate. A detection line and a quality control line are arranged on the nitrocellulose membrane. The gold standard cushion is coated with dexamethasone monoclonal antibodies which are marked with colloidal gold and can be recognized. The detection line is coated with dexamethasone coating antigens. The quality control line is coated with goat-ant-mouse IgG. The invention further discloses a preparation method and application of the colloidal goldy test strip for detecting the dexamethasone residues in milk. The test strip can be used for analyzing the dexamethasone residues in milk, and the detection limit is 0.2 microgram per kilogram. The test strip has the advantages of being high in specificity, good in stability and the like.

Description

technical field [0001] The invention belongs to the technical field of immune analysis of drug residues in animal products, and in particular relates to a colloidal gold immunochromatographic test strip for rapidly detecting dexamethasone residues in milk. Background technique [0002] Dexamethasone is one of the commonly used glucocorticoid drugs in clinical practice. It is often used together with other drugs. It has anti-inflammatory, anti-toxic and anti-allergic effects. It can also improve feed conversion rate and increase animal weight. The irrational use of such drugs may lead to residues of glucocorticoids in animal-derived foods, which constitutes a potential hazard to human health. [0003] Studies have shown that if you take too much glucocorticoid drugs, it can cause substance metabolism and water and salt metabolism disorders, adrenal hyperfunction syndrome, induce cataracts, osteoporosis and cardiovascular system complications. Therefore, both my country and t...

Claims

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Application Information

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IPC IPC(8): G01N33/94G01N33/74G01N33/577G01N33/558C07K16/26C12R1/91
CPCG01N33/94C07K16/26G01N33/558G01N33/577G01N33/74G01N2333/575
Inventor 周琪冯亮陈建军
Owner WUHAN SHANGCHENG BIOTECH
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