A kind of composite biocontrol agent containing seaweed matrix and its preparation method and application

Abstract

The invention discloses a composite biocontrol agent containing seaweed matrix, a preparation method and application. The biocontrol agent includes seaweed residue, Bacillus subtilis 2‑1, and Bacillus amyloliquefaciens 9‑2, wherein the ratio of seaweed residue to Bacillus fermentation broth is 2 to 8:1 (w / v), and Bacillus subtilis The dosage ratio of 2‑1 to Bacillus amyloliquefaciens 9‑2 is 1:1~5. The preparation method includes: inoculating and fermenting, preparing a biocontrol agent containing seaweed matrix, drying and packing for standby use. The composite biocontrol agent of the present invention not only has a strong inhibitory effect on the root rot pathogens of various Chinese medicinal materials such as Salvia miltiorrhiza and Araceae, but also has a better control effect on root rot of vegetable crops, and the bacteriostatic effect is better than that of a single plant bacteria.

Description

technical field

[0001] The invention belongs to the field of biocontrol fungi, in particular to a compound biocontrol fungus containing seaweed matrix, its preparation method and application. Background technique

[0002] Biocontrol Bacillus has many types, wide distribution, broad antibacterial spectrum, and obvious disease resistance and yield increase effect, which is superior to other antagonistic microorganisms. At present, there have been reports of its successful use in plant disease control at home and abroad. There are 14 kinds of microbial pesticides that have been registered in my country to prevent and control soil-borne diseases, of which 6 kinds use Bacillus as the biocontrol bacteria, which are mainly used for the control of rice and wheat sheath blight. In recent years, they have been rapidly applied to tomato Various crop and / or fruit and vegetable diseases such as bacterial wilt, cotton verticillium wilt, pepper wilt, watermelon wilt, tobacco black shank, ri...

Examples

Embodiment 1

[0036]A kind of composite biocontrol bacterium agent containing seaweed substrate, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 2: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:1. Both Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 were preserved on May 29, 2014 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation address is: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101. Among them, the preservation number of Bacillus subtilis 2-1 is: CGMCC No. 9256, and the classification name is: Bacillus subtilis (Bacillus subtilis); the preservation number of Bacillus amyloliquefaciens 9-2 is: CGMCC No. 9257, and the classificati...

Embodiment 2

[0052] A kind of composite biocontrol bacterium agent containing seaweed matrix, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 8: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:2.

[0053] A preparation method of a composite biocontrol agent containing seaweed matrix, comprising the following steps:

[0054] 1) Inoculation fermentation

[0055] a. Under aseptic operation conditions, use a pipette gun to transfer the biocontrol strains 2-1 and 9-2 preserved in glycerol tubes according to different dosages to the glass plates equipped with the first medium, and incubate at 37°C for 18 hours Activation of bacteria;

[0056] The first medium formula is: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, agar 20g / L, pH value is 7.5;

[0057] b. Inoculate the activated strain...

Embodiment 3

[0069] A kind of composite biocontrol bacterium agent containing seaweed matrix, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 4: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:3.

[0070] A preparation method of a composite biocontrol agent containing seaweed matrix, comprising the following steps:

[0071] 1) Inoculation fermentation

[0072] a. Under aseptic operation conditions, inoculate the biocontrol strains 2-1 and 9-2 preserved in glycerol tubes according to different dosages on the glass plates containing the first medium with a pipette gun, and incubate at 37°C for 18 hours Activation of bacteria;

[0073] The first medium formula is: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, agar 20g / L, pH value is 7.5;

[0074] b. Inoculate the activated strains in...

technical field

[0001] The invention belongs to the field of biocontrol fungi, in particular to a compound biocontrol fungus containing seaweed matrix, its preparation method and application. Background technique

[0002] Biocontrol Bacillus has many types, wide distribution, broad antibacterial spectrum, and obvious disease resistance and yield increase effect, which is superior to other antagonistic microorganisms. At present, there have been reports of its successful use in plant disease control at home and abroad. There are 14 kinds of microbial pesticides that have been registered in my country to prevent and control soil-borne diseases, of which 6 kinds use Bacillus as the biocontrol bacteria, which are mainly used for the control of rice and wheat sheath blight. In recent years, they have been rapidly applied to tomato Various crop and / or fruit and vegetable diseases such as bacterial wilt, cotton verticillium wilt, pepper wilt, watermelon wilt, tobacco black shank, ri...

Examples

Embodiment 1

[0036]A kind of composite biocontrol bacterium agent containing seaweed substrate, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 2: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:1. Both Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 were preserved on May 29, 2014 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, and the preservation address is: Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101. Among them, the preservation number of Bacillus subtilis 2-1 is: CGMCC No. 9256, and the classification name is: Bacillus subtilis (Bacillus subtilis); the preservation number of Bacillus amyloliquefaciens 9-2 is: CGMCC No. 9257, and the classificati...

Embodiment 2

[0052] A kind of composite biocontrol bacterium agent containing seaweed matrix, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 8: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:2.

[0053] A preparation method of a composite biocontrol agent containing seaweed matrix, comprising the following steps:

[0054] 1) Inoculation fermentation

[0055] a. Under aseptic operation conditions, use a pipette gun to transfer the biocontrol strains 2-1 and 9-2 preserved in glycerol tubes according to different dosages to the glass plates equipped with the first medium, and incubate at 37°C for 18 hours Activation of bacteria;

[0056] The first medium formula is: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, agar 20g / L, pH value is 7.5;

[0057] b. Inoculate the activated strain...

Embodiment 3

[0069] A kind of composite biocontrol bacterium agent containing seaweed matrix, this biocontrol bacterium agent comprises seaweed residue, bacillus subtilis 2-1, bacillus amyloliquefaciens 9-2, wherein the consumption ratio of seaweed residue and bacillus fermented liquid is 4: 1 (w / v), the dosage ratio of Bacillus subtilis 2-1 and Bacillus amyloliquefaciens 9-2 is 1:3.

[0070] A preparation method of a composite biocontrol agent containing seaweed matrix, comprising the following steps:

[0071] 1) Inoculation fermentation

[0072] a. Under aseptic operation conditions, inoculate the biocontrol strains 2-1 and 9-2 preserved in glycerol tubes according to different dosages on the glass plates containing the first medium with a pipette gun, and incubate at 37°C for 18 hours Activation of bacteria;

[0073] The first medium formula is: peptone 10g / L, yeast extract powder 5g / L, sodium chloride 10g / L, agar 20g / L, pH value is 7.5;

[0074] b. Inoculate the activated strains in...

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