Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Effective part of perilla leaf resisting hyperuricemia and preparation method and application thereof

A technology of hyperuricemia and effective parts, applied in the field of medicine, can solve the problems of no anti-hyperuricemia effective parts reported in the literature

Active Publication Date: 2015-11-18
QINGDAO UNIV
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching the prior art literature, there is no literature report on the anti-hyperuricemia effective part of perilla leaf water extract

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Effective part of perilla leaf resisting hyperuricemia and preparation method and application thereof
  • Effective part of perilla leaf resisting hyperuricemia and preparation method and application thereof
  • Effective part of perilla leaf resisting hyperuricemia and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Screening of Effective Parts of Perilla Leaf for Anti-hyperuricemia

[0029] Preparation of perilla leaf extract and isolated parts: 4Kg of perilla leaf medicinal material, add 35 times the amount of water to decoct three times, 2 hours each time, filter, combine the filtrates, concentrate to 2000mL, add 80% ethanol to adjust the alcohol concentration to 60 %, let stand for 24 hours, filter, and recover ethanol under reduced pressure to obtain 629g of extract (PFLT). Add 600g of the extract to 2000mL of water to dissolve, shake and extract with n-butanol 4 times, 2000mL each time, combine the extracts and recover under reduced pressure to obtain 128g of n-butanol separation parts (PFLTB) and 485g of water separation parts (PFLTH). 120 g of n-butanol separation fractions were chromatographed on a macroporous adsorption resin HPD-600 column, and eluted with water and 70% ethanol to obtain 21 g of water elution fractions (PFLTB-A) and 96 g of 70% ethanol elution fractions ...

Embodiment 2

[0036] HPLC method for determination of caffeic acid, caffeic acid vinyl ester, rosmarinic acid, rosmarinic acid methyl ester and scutellarin in effective antihyperuricemia parts of perilla leaves

[0037] Chromatographic conditions and system suitability test

[0038] Octadecylsilane bonded silica gel as filler; methanol as mobile phase A, 0.1 phosphoric acid solution as mobile phase B, linear gradient elution: 0min (A30%, B70%, volume ratio) → 10min (A30% , B70%, volume ratio) → 25min (A40%, B60%, volume ratio) → 50min (A40%, B60%); flow rate: 1.0mL min -1 ; The detection wavelength is 330nm. The number of theoretical plates is calculated based on the peak of rosmarinic acid, not less than 4000.

[0039] Solution preparation

[0040] Preparation of reference solution

[0041] Accurately weigh the appropriate amount of caffeic acid, caffeic acid vinyl ester, rosmarinic acid, rosmarinic acid methyl ester and scutellarin reference substance, add methanol to make each 1mL co...

Embodiment 3

[0047] 50Kg of perilla leaves (origin in Changchun, Jilin), add 35 times the amount of water to decoct three times, 2 hours each time, filter, combine the filtrates, concentrate to 25L, add 80% ethanol to adjust the alcohol concentration to 60%, and let it stand for 24 hours , filtered, recover ethanol under reduced pressure to obtain extract, add 25L of water to the extract to dissolve, shake and extract 4 times with n-butanol, 25L each time, combine the extracts, recover under reduced pressure to obtain the separation part of n-butanol, n-butanol Add water to dissolve the alcohol separation part, go through macroporous adsorption resin column HPD-600 chromatography, fill the volume of macroporous adsorption resin HPD-600 to 25L, elute with water until the eluent is nearly colorless, discard the eluent, and wash with 70% ethanol After elution until the eluate was nearly colorless, the eluate was collected, concentrated under reduced pressure, and dried to obtain 1317 g of effe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an effective part of perilla leaf resisting hyperuricemia and a preparation method and application thereof. The effective part takes the perilla leaf as a raw material, and is prepared by the steps of extracting with water, concentrating, ethanol precipitation, reducing pressure, recycling ethanol and obtaining an extractive; performing liquid-liquid partition of n-butyl alcohol and water on the extractive to obtain the separated part of the n-butyl alcohol; purifying the separated part of the n-butyl alcohol by macroporous resin to obtain the effective part of perilla leaf. The sum of the weight of caffeic acid, caffeic acid vinyl ester, rosmarinic acid and methyl rosmarinate accounts for 50 to 70 percent of the effective part, and the effective part contains 5 to 10 percent of scutellarin. A modern separation method and pharmacological activity screening are combined to determine the best effective part, so that the ingredient of the obtained effective part is more clear, the content of the active components is high; the result of experiment in vitro and vivo shows that the effective part of perilla leaf resisting hyperuricemia can inhabit the xanthine oxidase activity to achieve the effect of reducing trioxypurine.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the anti-hyperuricemia effective part of perilla leaf and a preparation method thereof, and also relates to the use of the anti-hyperuricemia effective part of perilla leaf in preparing products for treating hyperuricemia. Background technique [0002] Hyperuricemia is a group of heterogeneous diseases caused by purine metabolic disorders or (and) decreased uric acid excretion. It is an important biochemical basis for gout. It is a disease that seriously affects public health. Hyperuricemia not only seriously affects the patient's quality of life, but even threatens the life of the patient, seriously threatens the state of public health, and brings a heavy economic burden to the society. Xanthine oxidase, as the key enzyme of uric acid production, is the target of uric acid-lowering drugs. Allopurinol and febuxostat are clinically used xanthine oxidase inhibitors, but skin allergies, hepat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/535A61P19/06A61K31/7048A61K31/192A61K31/216A61K31/353
Inventor 王威韩立春师海波刘小红刘洋
Owner QINGDAO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com