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Aspergillus oryzae as well as culture method and application thereof

A culture method, the technology of Aspergillus oryzae, applied in the field of microorganisms, can solve the problems of low conversion rate, low enzyme activity, and difficult purification, and achieve the effects of reducing difficulty and cost, improving use efficiency, and reducing production cost

Active Publication Date: 2015-12-02
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current domestic and foreign production methods of fructooligosaccharides are mainly strain fermentation and enzyme conversion. Strain fermentation refers to the direct fermentation of sucrose solution to produce fructooligosaccharides with the strains producing β-fructosyltransferase. The disadvantages of this method The purity of the produced fructooligosaccharide is not high, and subsequent purification is difficult
The enzymatic conversion method refers to cultivating enzyme-producing bacteria first, and then extracting β-fructosyltransferase for enzymatic conversion to produce fructo-oligosaccharides. The current problem of this method is that the extracted enzyme activity is low, and the high content of side effects in the reaction is affected during the conversion process. The influence of the product glucose causes the conversion rate to be low and the production cost to remain high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A strain of Aspergillus oryzae (Aspergillus oryzae) BYCY-03 was preserved in the General Microbiology Center of China Committee for Microorganism Culture Collection on July 15, 2014, with the preservation number CGMCCNo.9450. Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology, Chinese Academy of Sciences.

[0034] The screening process of the above-mentioned Aspergillus oryzae (Aspergillusoryzae) BYCY-03 is as follows:

[0035] (1) Enrichment culture

[0036] Select the soil near the fructooligosaccharide production workshop of Bailong Chuangyuan, Dezhou, Shandong, remove the topsoil with a small shovel, take about 10g of soil 5-15cm above the ground, dilute 10 times with sterile water, add PDA medium for enrichment culture , medium composition:

[0037] 200 grams of potatoes, 20 grams of glucose, 15-20 grams of agar, 1000 ml of deionized water, 0.2% ammonium nitrate; 0.1% ammonium sulfate; 0.1% dipotassium hydrogen pho...

Embodiment 2

[0053] The cultivation method of aspergillus oryzae (Aspergillusoryzae) BYCY-03 described in embodiment 1, the steps are as follows:

[0054] (1) Take Aspergillus oryzae BYCY-03 and inoculate it in PDA medium, and activate and cultivate it for 30 hours at 30°C to obtain an activated strain;

[0055] (2) Take the activated bacterial strain prepared in step (1), inoculate it in the seed culture medium, and proliferate and cultivate it for 30 hours under the condition of 30° C. to obtain the seed liquid;

[0056] The seed medium components are as follows:

[0057] Add 2 grams of soluble starch, 0.1 grams of potassium dihydrogen phosphate, 0.05 grams of magnesium sulfate, 0.05 grams of ammonium sulfate, 2 grams of agar, and 2 grams of agar to 100 ml of bean cake extract juice, and the natural pH

[0058] The preparation method of the bean cake extract juice: 100 grams of bean cake, add 500 ml of water, soak for 4 hours, boil for 3 to 4 hours, filter naturally with gauze, take the...

Embodiment 3

[0064] The cultivation method of aspergillus oryzae (Aspergillusoryzae) BYCY-03 described in embodiment 1, the steps are as follows:

[0065] (1) Take Aspergillus oryzae (Aspergillusoryzae) BYCY-03 and inoculate it in PDA medium, and activate and cultivate it for 20 hours at 35°C to obtain an activated strain;

[0066] (2) Take the activated bacterial strain obtained in step (1), inoculate it in the seed culture medium, and proliferate and cultivate it for 20 hours under the condition of 35° C. to obtain the seed solution;

[0067] The seed medium components are as follows:

[0068] Add 2 grams of soluble starch, 0.1 grams of potassium dihydrogen phosphate, 0.05 grams of magnesium sulfate, 0.05 grams of ammonium sulfate, 2 grams of agar, and 2 grams of agar to 100 ml of bean cake extract juice, and the natural pH

[0069] The preparation method of the bean cake extract juice: 100 grams of bean cake, add 500 ml of water, soak for 4 hours, boil for 3-4 hours, filter naturally w...

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Abstract

The invention relates to an Aspergillus oryzae as well as a culture method and application thereof. The Aspergillus oryzae BYCY-03 is preserved in the general microorganism center of the China committee for culture collection of microorganisms on July 15, 2014, and the preservation number is CGMCC No.9450. The invention further relates to the culture method and application of Aspergillus oryzae. According to the Aspergillus oryzae BYCY-03, the enzyme activity reaches 1,200 U / ml which is increased by 40% or above compared with the conventional beta-fructosyl transferase; the Aspergillus oryzae BYCY-03 also has the property of resisting glucose; when the Aspergillus oryzae is applied to fructooligosaccharide production, the capability of converting sucrose into fructooligosaccharide can be greatly improved, and the production cost can be obviously reduced.

Description

technical field [0001] The invention relates to a strain of Aspergillus oryzae and its cultivation method and application, belonging to the technical field of microbes. Background technique [0002] Aspergillus oryzae is a common species in the subphylum Deuteromycota, Hyphospora, Hyphospora, from the family Stemaceae, Aspergillus fungi. It is widely distributed, mainly in food, fermented food, spoiled organic matter and soil. It is the production strain of my country's traditional brewed food sauce and soy sauce. Amylase, protease, pectinase and kojic acid can also be produced. It will cause mildew in industrial and agricultural products such as grain. [0003] Fructo-oligosaccharides refer to carbohydrates formed by linking 2 to 5 fructosyl groups as chain links, a glucose group as the end group of the chain, and fructosyl→fructose linkage as the main skeleton. FOS is a natural active substance. The sweetness is 0.3-0.6 times that of sucrose. It not only maintains th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N11/14C12N11/12C12P19/18C12P19/12C12P19/04C12P19/00C12R1/69
Inventor 李方华窦宝德窦光鹏干昭波邵先豹
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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