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A strain of Aspergillus niger and its cultivation method and application

A cultivation method, the technology of Aspergillus niger, applied in the field of microorganisms, can solve the problems of low conversion rate, low enzyme activity, difficult purification, etc., and achieve the effects of reducing difficulty and cost, improving quality, and reducing production cost

Active Publication Date: 2017-01-25
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The current domestic and foreign production methods of fructooligosaccharides are mainly strain fermentation and enzyme conversion. Strain fermentation refers to the direct fermentation of sucrose solution to produce fructooligosaccharides with the strains producing β-fructosyltransferase. The disadvantages of this method The purity of the produced fructooligosaccharide is not high, and subsequent purification is difficult
The enzymatic conversion method refers to cultivating enzyme-producing bacteria first, and then extracting β-fructosyltransferase for enzymatic conversion to produce fructo-oligosaccharides. The current problem of this method is that the extracted enzyme activity is low, and the high content of side effects in the reaction is affected during the conversion process. The influence of the product glucose causes the conversion rate to be low and the production cost to remain high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A strain of Aspergillus niger (Aspergillus niger) BLCY-02 was preserved on July 15, 2014 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, with the preservation number CGMCC No.9449, address: No. 1, Beichen West Road, Chaoyang District, Beijing 3 Institute of Microbiology, Chinese Academy of Sciences.

[0033] The screening process of the above-mentioned Aspergillus niger (Aspergillus niger) BLCY-02 is as follows:

[0034] (1) Enrichment culture

[0035] Select the soil near the fructooligosaccharide production workshop in Bailong Chuangyuan, Dezhou, Shandong, remove the topsoil with a small shovel, take about 10g of soil at a distance of 5-15cm from the ground, dilute 10 times with sterile water, and add Cha's medium for enrichment Cultivation, Cha's medium composition: sodium nitrate 0.3%; dipotassium hydrogen phosphate 0.1%; magnesium sulfate (MgSO4 7H2O) 0.05%; potassium chloride 0.05%; ferrous sulfate 0.001%; ​​sucrose...

Embodiment 2

[0046] The cultivation method of the Aspergillus niger (Aspergillus niger) BLCY-02 described in embodiment 1, the steps are as follows:

[0047] (1) Aspergillus niger (Aspergillus niger) BLCY-02 was inoculated in PDA medium, and activated and cultured for 30 hours at 30°C to obtain an activated strain;

[0048] (2) Take the activated bacterial strain prepared in step (1), inoculate it in the seed culture medium, and proliferate and cultivate it for 30 hours under the condition of 30° C. to obtain the seed solution;

[0049] Described seed culture medium component is as follows, is weight percent:

[0050] Sodium nitrate 0.3%; Dipotassium hydrogen phosphate 0.1%; Magnesium sulfate (MgSO 4 ·7H 2 O) 0.05%; yeast extract 1%; sucrose 5%, surplus water, pH is 6.0;

[0051] (3) Take the seed solution prepared in step (2), inoculate it in the fermentation medium at a ratio of 1% by volume, and expand the cultivation for 35 hours at 30° C. to obtain the bacterial cell fermentation s...

Embodiment 3

[0056] The cultivation method of the Aspergillus niger (Aspergillus niger) BLCY-02 described in embodiment 1, the steps are as follows:

[0057] (1) Aspergillus niger (Aspergillus niger) BLCY-02 was inoculated in PDA medium, and activated and cultured for 20 hours at 38°C to obtain an activated strain;

[0058] (2) Take the activated bacterial strain obtained in step (1), inoculate it in the seed culture medium, and proliferate and cultivate it for 20 hours under the condition of 38° C. to obtain the seed solution;

[0059] Described seed culture medium component is as follows, is weight percent:

[0060] Sodium nitrate 0.3%; Dipotassium hydrogen phosphate 0.1%; Magnesium sulfate (MgSO 4 ·7H 2 O) 0.05%; yeast extract 1%; sucrose 5%, balance water, pH is 4.0;

[0061] (3) Take the seed liquid prepared in step (2), inoculate it in the fermentation medium at a ratio of 10% by volume, and expand the cultivation for 20 hours at 38° C. to obtain the bacterial cell fermentation li...

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Abstract

The invention relates to an Aspergillus niger and a cultivation method and application thereof. The Aspergillus niger BLCY-02 is collected in the China General Microbiological Culture Collection Center on 15th, July in 2014 and has the collection number of CGMCC No.9449. The invention also relates to a cultivation method and application of the Aspergillus niger.

Description

technical field [0001] The invention relates to a strain of Aspergillus niger and its cultivation method and application, belonging to the technical field of microorganisms. Background technique [0002] Aspergillus niger, Deuteromycotina, Hyphomycetes, Hyphospora, Polygonaceae, a common species in the genus Aspergillus. Widely distributed in food, plant products and soils all over the world. It is an important fermentation industry strain, which can produce amylase, acid protease, cellulase, pectinase, glucose oxidase, citric acid, gluconic acid and gallic acid, etc. Some strains also convert hydroxyprogesterone to androsene. The optimum temperature for growth is 37°C, and the minimum relative humidity is 88%, which can cause mildew in grains with high moisture content and mildew in other industrial equipment. [0003] Fructo-oligosaccharides refer to carbohydrates formed by linking 2 to 5 fructosyl groups as chain links, a glucose group as the end group of the chain, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N9/10C12P19/18C12R1/685
Inventor 干昭波邵先豹杨腾腾窦光朋贾玉秋
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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