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Method and device for rapid propagation of virus-free potato plantlets in test tubes

A technology for test-tube seedlings and potatoes, which is applied in the field of rapid propagation methods and devices for virus-free potato test-tube seedlings, which can solve the problems of poor growth quality of test-tube seedlings, large amount of breeding chemicals, and large labor workload, and achieve fast operation speed and high pollution and scrap rate low quality effect

Active Publication Date: 2018-12-14
达州市农业科学研究所
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] The object of the present invention is to provide a method and device for rapid propagation of virus-free potato plantlets in test tubes, aiming to solve the problems of large dosage of medicines, high energy consumption, heavy labor and poor growth quality of test-tube plantlets in the existing indoor test-tube plantlets of virus-free potato plants. The problem

Method used

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  • Method and device for rapid propagation of virus-free potato plantlets in test tubes

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Embodiment 1

[0035] Select the test-tube seedling of No. 30 qualified potato varieties Qinyu through detoxification detection, according to the design method of the present invention, the water filter layer selects cobblestone for use, and the water filter layer selects filter sponge for use, and the nutrient matrix layer uses vermiculite to add perlite (2:1 ), on September 20, 2014, the test-tube seedlings were cut into single sections, and planted in the atomized cultivation tank. The cutting tool was sterilized with 0.1% KMnO4 solution, and after planting, 50% carbendazim wettable powder 500 Double liquid spray to prevent mold pollution, covered with 50% sunshade net for shade; planting density is 3cm×3cm, each planted 100 plants, repeated twice; spray according to the design method; remove the sunshade net after 7 days. After 20 days, the survival rate, number of leaves, plant height, and stem diameter were investigated, and the arithmetic mean was used for the data. At the same time i...

Embodiment 2

[0047] Select the test-tube seedlings of the potato variety "Da Shu No. 1" qualified through the detoxification test, according to the design method of the present invention, the water filter layer is selected from cobblestones, the water filter layer is selected from filter sponges, and the nutrient matrix layer is selected from vermiculite plus perlite, vermiculite. The volume ratio of stone to perlite is 2:1. On February 20, 2015, the test-tube seedlings were cut into single sections and planted in the atomized cultivation tank. The cutting tools were sterilized with 0.1% KMnO4 solution. Spray 500 times with 58% formazan phosphorus manganese zinc wettable powder, then cover the film, remove it after 5 days, spray according to the design method, planting density is 3cm×3cm, and plant 2000 plants. After 20 days of investigation, the average survival rate was 98.5%, the average number of leaves was 7.22, the average plant height was 6.75cm, and the average stem diameter was 1.0...

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Abstract

The invention discloses a virus-free potato test tube plantlet rapid propagation method and apparatus. A test tube plantlet is sheared into 1 to 2 pieces and planted into nutrient substrate of the virus-free potato test tube plantlet rapid propagation apparatus; test tube plantlet is managed; when growing to 5 to 10cm, the test tube plantlet is continued to be sheared for propagation; the shearing propagation is circulated according to the above method; when enough virus-free plantlets are propagated, plantation is properly conducted; and virus-free source breeder seeds are produced. During the propagation, the virus-free potato test tube plantlet is sheared into single piece and then directly planted on the soilless substrate; quick operation speed and low pollution disability rate can be achieved; the plantation can be conducted in the substrate cultivation groove, so the plantation is communicated with the external air; natural photosynthesis is employed, so seeds can well grow and high yield of production source can be acquired; the virus-free potato test tube plantlet can be planted in the cultivation grooves in a greenhouse; after the rapid propagation apparatus is well set, mist is automatically sprayed at regular time; and large manpower and material resources can be reduced.

Description

technical field [0001] The invention belongs to the field of potato production, in particular to a method and device for rapid propagation of virus-free potato tube seedlings. Background technique [0002] China is the most populous country in the world, and the contradiction between people and land is prominent. Food issues are critical. Potato is a rare high-yielding crop in the world. It has excellent characteristics such as comprehensive nutrition, wide adaptability, strong stress resistance, high and stable yield, long industrial chain, and dual use of grain, vegetable and feed industry raw materials. It is the fourth largest food crop in the world and the first in China. five major food crops. China's potato planting area and total output both rank first in the world, but the yield per unit area is relatively low. An important factor affecting the low yield of potato in China is the viral degradation of potato. Potatoes have an average yield reduction of 30%-80% af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00A01G31/02
CPCA01G31/00A01G31/02Y02P60/21
Inventor 胡振兴赵思毅杨小丽张玲徐建俊高龙梅李薇刘小英贾荟芹邹洁
Owner 达州市农业科学研究所
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