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Optimized separation method for human intestine probiotics

A technology of intestinal probiotics and separation method is applied in the field of optimal separation of human intestinal probiotics, which can solve the problems of slow separation of probiotics and other problems.

Active Publication Date: 2015-12-23
GUANGZHOU KANGZE MEDICAL TECH CO LTD +1
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention proposes an optimized separation method for human intestinal probiotics, which solves the problem of slow separation of probiotics

Method used

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  • Optimized separation method for human intestine probiotics
  • Optimized separation method for human intestine probiotics
  • Optimized separation method for human intestine probiotics

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Embodiment Construction

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0048] The inventors optimized and separated human intestinal probiotics according to the content provided by the present invention. The specific separation method is as follows:

[0049] Step 1, preparation of basal medium: prepare mucin liquid medium and mucin solid medium, wherein the mucin in the mucin liquid medium and the mucin solid medium is purified mucin, wherein the mucin The purification method is as follows:

[0050] Reagent preparation:

[0051] Abs...

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Abstract

The invention provides an optimized separation method for human intestine probiotics. The optimized separation method comprises the following steps that 1 basic medium preparing, wherein a mucoprotein liquid medium and a mucroprotein solid medium are prepared; 2 screening, wherein a feces specimen is collected and inoculated in the mucroprotein liquid medium for anaerobic culturing to obtain bacterium liquid, the bacterium liquid is taken as a template, a PCR experiment is performed, the bacterium liquid in a PCR positive reaction tube is selected to be inoculated in the mucroprotein solid medium for anaerobic culturing to obtain a bacterium colony, the bacterium colony on the mucroprotein solid medium is inoculated onto a chocolate plate for anaerobic culturing to obtain a bacterium colony, the bacterium colony on the chocolate plate is inoculated onto a Columbia blood plate for purification culturing to obtain a bacterium colony, and PCR sequencing verification is performed on the bacterium colony on the Columbia blood plate through a 16sRNA universal primer to separate the human intestine probiotics. The optimized separation method for the human intestine probiotics is practical, simple, visualized and convenient.

Description

technical field [0001] The invention relates to the field of isolation of human intestinal microorganisms, in particular to an optimized isolation method of human intestinal probiotics. Background technique [0002] Probiotics are closely related to human health. At present, there is no mature and convenient method for isolating and purifying probiotics in the field of microorganisms. In 2004, the first C-IFH strain was isolated in Europe and named Akkermansiamuciniphila. The rate of this strain in European adults is about 100%, and it has obvious weight loss and hypoglycemic effects. The method they used to isolate this bacterium was complicated, and the technology they designed was cumbersome and expensive, so the subsequent pure bacteria of other subtypes of this bacterium have not been reported so far. How to quickly isolate and purify such related bacteria has become a key technical breakthrough in exploring the correlation between C-IFH bacteria and diseases. Conte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02
CPCC12N1/20C12Q1/68C12N1/02
Inventor 彭永正易江丰郭先凤
Owner GUANGZHOU KANGZE MEDICAL TECH CO LTD
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