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An optimized isolation method of human intestinal probiotics

A technology of intestinal probiotics and separation method, which is applied in the field of optimized separation of human intestinal probiotics, and can solve the problems of slow separation of probiotics

Active Publication Date: 2019-01-11
GUANGZHOU KANGZE MEDICAL TECH CO LTD +1
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention proposes an optimized separation method for human intestinal probiotics, which solves the problem of slow separation of probiotics

Method used

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  • An optimized isolation method of human intestinal probiotics
  • An optimized isolation method of human intestinal probiotics
  • An optimized isolation method of human intestinal probiotics

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Embodiment Construction

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0048] The inventors optimized and separated human intestinal probiotics according to the content provided by the present invention. The specific separation method is as follows:

[0049] Step 1, preparation of basal medium: prepare mucin liquid medium and mucin solid medium, wherein the mucin in the mucin liquid medium and the mucin solid medium is purified mucin, wherein the mucin The purification method is as follows:

[0050] Reagent preparation:

[0051] Abs...

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Abstract

The present invention proposes an optimized separation method of human intestinal probiotics, comprising the following steps: step 1, preparation of basal medium: preparing mucin liquid medium and mucin solid medium; step 2: screening: collecting stool samples And inoculated in the mucin liquid culture medium, anaerobic culture to obtain the bacterial liquid, take the bacterial liquid as a template to carry out PCR experiment, select the bacterial liquid in the PCR reaction positive tube and inoculate it on the mucin solid medium to anaerobically cultivate a colony , the colonies on the mucin solid medium were inoculated on the chocolate plate for anaerobic culture, and the colonies on the chocolate plate were inoculated on the Columbia blood plate to purify and cultivate the colonies, and the colonies on the Columbia blood plate were passed through the 16sRNA universal primer PCR sequencing verification was carried out to isolate human intestinal probiotics. The optimized separation method of human intestinal probiotics of the present invention is practical, simple, intuitive and convenient.

Description

technical field [0001] The invention relates to the field of isolation of human intestinal microorganisms, in particular to an optimized isolation method of human intestinal probiotics. Background technique [0002] Probiotics are closely related to human health. At present, there is no mature and convenient method for isolating and purifying probiotics in the field of microorganisms. In 2004, the first C-IFH strain was isolated in Europe and named Akkermansia muciniphila. The rate of this strain in European adults is about 100%, and it has obvious weight loss and hypoglycemic effects. The method they used to isolate this bacterium was complicated, and the technology they designed was cumbersome and expensive, so the subsequent pure bacteria of other subtypes of this bacterium have not been reported so far. How to quickly isolate and purify such related bacteria has become a key technical breakthrough in exploring the correlation between C-IFH bacteria and diseases. Cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/02
CPCC12N1/20C12Q1/68C12N1/02
Inventor 彭永正易江丰郭先凤
Owner GUANGZHOU KANGZE MEDICAL TECH CO LTD
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