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A method for establishing a screening library for identifying sources of soil samples
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A soil sample and source technology, applied in soil material testing, material inspection products, etc., can solve problems such as undiscovered research reports
Active Publication Date: 2017-10-20
UNIV OF JINAN
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[0004] At present, there are relatively simple applications of soil as trace evidence in forensic medicine in foreign countries, but no relevant research reports have been found in China.
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Embodiment 1
[0074] Source of soil samples: Soil samples collected from wheat fields in 87 regions in Shandong Province before wheat sowing in autumn. Numbers S1-S87.
[0075] A method for establishing a screening library for identifying soil sample sources, the process is as follows figure 1 shown, including the following steps:
[0076] (1) Take a soil sample from a known source, put it in a dry and clean glass petri dish, use the Mensell color card to detect the color of the sample, compare the soil sample with a card with a standard color scale, and detect the soil color. classify the results;
[0077] Taking the Mensell standard color card as a comparison, when the main color of the sample is red, the sample is identified as red soil; when the main color of the sample is yellow, the sample is identified as yellow soil; when the main color of the sample is black, the sample is identified for black soil;
[0078] (2) adopt standard pipette method to detect the particle diameter R of...
[0137] Number the randomly drawn samples as X1, X2, X3, X4, X5, take samples, and analyze these 5 samples according to the above method, the steps are as follows:
[0138] (1) Get soil samples X1, X2, X3, X4, X5, use the Mensell color card to detect the sample color, compare the soil sample with the card with the standard color scale, detect the soil color, and classify according to the test results;
[0139] Taking the Mensell standard color card as a comparison, when the main color of the sample is red, the sample is identified as red soil; when the main color of the sample is yellow, the sample is identified as yellow soil; when the main color of the sample is black, the sample is identified for black soil;
[0140] (II) adopt standard pipette method to detect the particle diameter of sample, classify according to test result;
[0141] When the test result is 50-2000 μm, it...
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Abstract
The invention relates to a method for establishing a screening library for identifying sources of soil samples, comprising the following steps: (1) taking soil samples of known sources, and using the Mensell color card to detect the color of the samples for classification; (2) using a standard straw method Detect the particle size of the sample and classify it; (3) use a scanning electron microscope to detect and classify the scanning image of the sample; (4) use the energy spectrum method to detect the soil sample and group it into a library; (5) use the infraredchromatography to detect the sample Detect, analyze and classify the compounds contained in the soil; (6) extract the DNA in the sample for PCR with fluorescent primers and classify; (7) according to the different classifications of the source of the sample, establish a screening library for identifying the source of soil samples. The invention can clearly distinguish the soil in different regions, thereby providing an effective source determination method for the soil source of the sample to be tested.
Description
technical field [0001] The invention relates to a method for establishing a screening library for identifying sources of soil samples, and belongs to the technical field of soil detection and analysis. Background technique [0002] Soil is the main body of the earth's surface. It is a relatively loose substance formed by rocks after long-term natural erosion. It is mainly composed of many layers of minerals with different thicknesses. There are many kinds of components in the soil, and the structures, properties and functions of various chemical components are very complex, which can provide abundant nutrients and sufficient water for the growth of plants on the earth's surface and the exchange of energy in the ecological cycle. Soils of different origins have different specificities because soils are constantly subjected to various physical, chemical, and biological factors such as climate, early-formed textures, microbial environments, and human activities. [0003] The r...
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