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Recombinant human type 3 adenovirus as well as preparation method and application thereof

An adenovirus and epitope technology, applied in the fields of recombinant human adenovirus type 3 and its preparation and application, can solve the problem that the prevention of hand and foot disease cannot be effectively prevented, and achieve the effect of strengthening immunity

Inactive Publication Date: 2016-01-06
东莞市第八人民医院
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Problems solved by technology

[0004] Chinese published patent 201310043885.9 discloses a recombinant human type 3 adenovirus and its preparation method and application, and discloses that EV71 neutralizing epitopes SP70 and SP55 are inserted into the recombinant human type 3 adenovirus, which can prevent EV71 infection and HAdv3 infection at the same time prevention, but it is still unable to effectively prevent hand-foot disease

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  • Recombinant human type 3 adenovirus as well as preparation method and application thereof

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Embodiment Construction

[0017] In order to facilitate the understanding of those skilled in the art, the present invention will be further described below in conjunction with the examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The specific details not mentioned in the following examples Experimental methods are usually carried out in accordance with conventional experimental methods.

[0018] Hadv3-EV71 described in this embodiment uses the HRV1 region of the hexon of the Hadv3 virus strain (full gene Genbank number: DQ099432) inserted into the EV71-08-02 virus strain (full genome sequence Genbank number: FJ360545) Antigen epitope sequence, amino acid sequence without mutation.

[0019] The backbone plasmid pBRAdSP70△E3GFP used in this example is the DNA sequence of SP70 (5'-tatcccacattcggagaacacaaacaggagaaagatcttgaatat-3') obtained by using the preparation method in the Chinese p...

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Abstract

The invention belongs to the technical field of recombinant virus genetic engineering, and in particular relates to a novel human enterovirus 71 (EV71)-coxackievirus A16 (CA16)-recombinant type 3 adenovirus (Had 3) candidate vaccine strain using recombinant human type 3 adenovirus as a vector, and a preparation method of the strain; hexon of the human type 3 adenovirus is simultaneously interpolated with a neutralizing epitope SP70 of the EV71 and a neutralizing epitope VP1-1 of the CA16, wherein the amino acid sequence of the neutralizing epitope SP70 of the EV71 is shown as YPTFGEHKQEKDLEYC and the amino acid sequence of the neutralizing epitope VP1-1 of the CA16 is shown as PKPTSRDSFAWQTAT. The recombinant human type 3 adenovirus is capable of simultaneously preventing EV71 and CA16, and the adenovirus has a significant effect on preventing hand-foot-and-mouth disease.

Description

technical field [0001] The invention belongs to the technical field of recombinant virus genetic engineering, and specifically relates to a new type of enterovirus 71 (humanenterovirus71, EV71)-Coxackievirus A16 (CoxackievirusA16, CA16)-recombinant 3 Type adenovirus (Had3) vaccine candidate strain and preparation method. Background technique [0002] Hand, foot and mouth disease (HFMD) is an acute infectious disease caused by human enterovirus, with fever and rash on the hands, feet and oral cavity or herpetic angina as the main clinical features. HFMD mainly occurs in children under 5 years old and is mainly caused by two pathogens, enterovirus 71 (humanenterovirus71, EV71) and Coxackievirus A16 (Coxackievirus A16, CA16). The disease has been reported all over the world in recent years. Although it can be treated clinically with drugs, the spread of the disease cannot be controlled, and large-scale infection cases will appear every year during the high incidence period. T...

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Application Information

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IPC IPC(8): C12N7/01C12N15/861A61K39/295A61K39/125A61P31/14C12R1/93
Inventor 蒋再学田新贵陆小梅骆庆明马强
Owner 东莞市第八人民医院
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